Strategic Combination of Isocratic and Gradient Elution for Simultaneous Separation of Polar Compounds in Traditional Chinese Medicines by HPLC
Joint Authors
Li, Yu-Sang
Tang, He-Bin
Lan, Wei
Zhang, Mei-Tuo
Ye, Xiao-Xue
Yang, Yan-Ling
Wu, Tsunghsueh
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-19
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
A simple high-performance liquid chromatography (HPLC) method for the simultaneous separation of the highly polar and weakly polar components of traditional Chinese medicines was developed via a strategic combination of isocratic and gradient elution methods.
Liu-Shen-Wan and Liu-Wei-Di-Huang-Wan were used as representative examples of traditional Chinese medicines.
This is the first time that 6 components of varying degrees of polarity in Liu-Shen-Wan had been successfully resolved in a single chromatographic run using an ultraviolet-visible detector with a fixed wavelength of 296 nm.
In contrast to conventional gradient separation methods, this novel method offered a viable route for separation of the highly and weakly polar fractions simultaneously, thus greatly reducing the time and cost of analysis.
This method therefore provides a more efficient way to determine the polar components present in traditional Chinese medicines.
It would find potential application in drug screening, drug authentication, and product quality control.
American Psychological Association (APA)
Zhang, Mei-Tuo& Ye, Xiao-Xue& Lan, Wei& Yang, Yan-Ling& Wu, Tsunghsueh& Li, Yu-Sang…[et al.]. 2018. Strategic Combination of Isocratic and Gradient Elution for Simultaneous Separation of Polar Compounds in Traditional Chinese Medicines by HPLC. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1176513
Modern Language Association (MLA)
Zhang, Mei-Tuo…[et al.]. Strategic Combination of Isocratic and Gradient Elution for Simultaneous Separation of Polar Compounds in Traditional Chinese Medicines by HPLC. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-6.
https://search.emarefa.net/detail/BIM-1176513
American Medical Association (AMA)
Zhang, Mei-Tuo& Ye, Xiao-Xue& Lan, Wei& Yang, Yan-Ling& Wu, Tsunghsueh& Li, Yu-Sang…[et al.]. Strategic Combination of Isocratic and Gradient Elution for Simultaneous Separation of Polar Compounds in Traditional Chinese Medicines by HPLC. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1176513
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176513