Development and Validation of a High-Performance Liquid Chromatographic Method for the Determination of Cinitapride in Human Plasma
Joint Authors
Kahsay, Getu
Eticha, Tadele
Thangabalan, Boovizhikannan
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-28
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
A precise and reliable reversed-phase high-performance liquid chromatographic method with ultraviolet detection was developed and validated to determine cinitapride in human plasma.
After liquid-liquid extraction, chromatographic separation was achieved on a Nucleosil C18 (25 cm × 4.6 mm, 5 µm) column with an isocratic elution consisting of 10 mM ammonium acetate (pH 5.2), methanol, and acetonitrile, 40 : 50 : 10, v/v/v.
The developed method was validated as per US FDA guidelines for its linearity, selectivity, sensitivity, precision, accuracy, and stability.
Satisfactory findings were obtained from the validation studies.
The linearity range of the method was 1 to 35 ng/mL while the extraction recovery of cinitapride in human plasma was more than 86%.
The percent coefficient of variation of both intraday and interday precision was ≤7.1%.
American Psychological Association (APA)
Thangabalan, Boovizhikannan& Kahsay, Getu& Eticha, Tadele. 2018. Development and Validation of a High-Performance Liquid Chromatographic Method for the Determination of Cinitapride in Human Plasma. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-5.
https://search.emarefa.net/detail/BIM-1176567
Modern Language Association (MLA)
Thangabalan, Boovizhikannan…[et al.]. Development and Validation of a High-Performance Liquid Chromatographic Method for the Determination of Cinitapride in Human Plasma. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-5.
https://search.emarefa.net/detail/BIM-1176567
American Medical Association (AMA)
Thangabalan, Boovizhikannan& Kahsay, Getu& Eticha, Tadele. Development and Validation of a High-Performance Liquid Chromatographic Method for the Determination of Cinitapride in Human Plasma. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-5.
https://search.emarefa.net/detail/BIM-1176567
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176567