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Determination of Fluoroquinolones in Pharmaceutical Formulations by Extractive Spectrophotometric Methods Using Ion-Pair Complex Formation with Bromothymol Blue
Joint Authors
Pham, Tien Duc
Nguyen, Trung Dung
Le, Hoc Bau
Dong, Thi Oanh
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-04
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
In this paper, we reported a new, simple, accurate, and precise extractive spectrophotometric method for the determination of fluoroquinolones (FQs) including ciprofloxacin (CFX), levofloxacin (LFX), and ofloxacin (OFX) in pharmaceutical formulations.
The proposed method is based on the ion-pair formation complexes between FQs and an anionic dye, bromothymol blue (BTB), in acidic medium.
The yellow-colored complexes which were extracted into chloroform were measured at the wavelengths of 420, 415, and 418 nm for CFX, LFX, and OFX, respectively.
Some effective conditions such as pH, dye concentration, shaking time, and organic solvents were also systematically studied.
Very good limit of detection (LOD) of 0.084 µg/mL, 0.101 µg/mL, and 0.105 µg/mL were found for CFX, LFX, and OFX, respectively.
The stoichiometry of the complexes formed between FQs and BTB determined by Job’s method of continuous variation was 1 : 1.
No interference was observed from common excipients occurred in pharmaceutical formulations.
The proposed method has been successfully applied to determine the FQs in some pharmaceutical products.
A good agreement between extractive spectrophotometric method with high-performance liquid chromatography mass spectrometry (HPLC-MS) for the determination of FQs in some real samples demonstrates that the proposed method is suitable to quantify FQs in pharmaceutical formulations.
American Psychological Association (APA)
Nguyen, Trung Dung& Le, Hoc Bau& Dong, Thi Oanh& Pham, Tien Duc. 2018. Determination of Fluoroquinolones in Pharmaceutical Formulations by Extractive Spectrophotometric Methods Using Ion-Pair Complex Formation with Bromothymol Blue. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1176592
Modern Language Association (MLA)
Nguyen, Trung Dung…[et al.]. Determination of Fluoroquinolones in Pharmaceutical Formulations by Extractive Spectrophotometric Methods Using Ion-Pair Complex Formation with Bromothymol Blue. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1176592
American Medical Association (AMA)
Nguyen, Trung Dung& Le, Hoc Bau& Dong, Thi Oanh& Pham, Tien Duc. Determination of Fluoroquinolones in Pharmaceutical Formulations by Extractive Spectrophotometric Methods Using Ion-Pair Complex Formation with Bromothymol Blue. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1176592
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176592