Rapid Screening and Quantitative Determination of Active Components in Qing-Hua-Yu-Re-Formula Using UHPLC-Q-TOFMS and HPLC-UV
Joint Authors
Wang, Xu
Shao, Xin
Zhao, Jie
Tao, Yi
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-11
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Qing-Hua-Yu-Re-Formula (QHYRF), a new herbal preparation, has been extensively used for treating diabetic cardiomyopathy.
However, the chemical constituents of QHYRF remain uninvestigated.
In the present study, rapid ultrahigh-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) was used to qualitatively analyze the components of QHYRF.
Qualitative detection was performed on a Kromasil C18 column through the gradient elution mode, using acetonitrile-water containing 0.1% formic acid.
Twenty-seven compounds were identified or tentatively characterized, including 12 phenolic acids, nine monoterpene glycosides, two flavonoids, three iridoids, and one unknown compound.
Among these, six compounds were confirmed by comparing with standards.
A high-performance liquid chromatography (HPLC) method was developed to simultaneously determine the following six active components in QHYRF: danshensu, paeoniflorin, acteoside, lithospermic acid, salvianolic acid B, and salvianolic acid C.
These HPLC chromatograms were monitored at 254, 280, and 320 nm.
The method was well validated with respect to specificity, linearity, limit of detection, limit of quantification, precision, stability, and recovery.
The HPLC-UV method was successfully applied to 10 batches of QHYRF.
American Psychological Association (APA)
Shao, Xin& Zhao, Jie& Wang, Xu& Tao, Yi. 2018. Rapid Screening and Quantitative Determination of Active Components in Qing-Hua-Yu-Re-Formula Using UHPLC-Q-TOFMS and HPLC-UV. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1176603
Modern Language Association (MLA)
Shao, Xin…[et al.]. Rapid Screening and Quantitative Determination of Active Components in Qing-Hua-Yu-Re-Formula Using UHPLC-Q-TOFMS and HPLC-UV. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1176603
American Medical Association (AMA)
Shao, Xin& Zhao, Jie& Wang, Xu& Tao, Yi. Rapid Screening and Quantitative Determination of Active Components in Qing-Hua-Yu-Re-Formula Using UHPLC-Q-TOFMS and HPLC-UV. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1176603
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176603