Automated and Reproducible Detection of Vascular Endothelial Growth Factor (VEGF) in Renal Tissue Sections
Joint Authors
Macedo, Nayana Damiani
Buzin, Aline Rodrigues
de Araujo, Isabela Bastos
Nogueira, Breno Valentim
Andrade, Tadeu Uggere
Endringer, Denise Coutinho
Lenz, Dominik
Source
Journal of Immunology Research
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-19
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Manual analysis of tissue sections, such as for pathological diagnosis, requires an analyst with substantial knowledge and experience.
Reproducible image analysis of biological samples is steadily gaining scientific importance.
The aim of the present study was to employ image analysis followed by machine learning to identify vascular endothelial growth factor (VEGF) in kidney tissue that had been subjected to hypoxia.
Methods.
Light microscopy images of renal tissue sections stained for VEGF were analyzed.
Subsequently, machine learning classified the cells as VEGF+ and VEGF- cells.
Results.
VEGF was detected and cells were counted with high sensitivity and specificity.
Conclusion.
With great clinical, diagnostic, and research potential, automatic image analysis offers a new quantitative capability, thereby adding numerical information to a mostly qualitative diagnostic approach.
American Psychological Association (APA)
Macedo, Nayana Damiani& Buzin, Aline Rodrigues& de Araujo, Isabela Bastos& Nogueira, Breno Valentim& Andrade, Tadeu Uggere& Endringer, Denise Coutinho…[et al.]. 2019. Automated and Reproducible Detection of Vascular Endothelial Growth Factor (VEGF) in Renal Tissue Sections. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1180705
Modern Language Association (MLA)
Macedo, Nayana Damiani…[et al.]. Automated and Reproducible Detection of Vascular Endothelial Growth Factor (VEGF) in Renal Tissue Sections. Journal of Immunology Research No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1180705
American Medical Association (AMA)
Macedo, Nayana Damiani& Buzin, Aline Rodrigues& de Araujo, Isabela Bastos& Nogueira, Breno Valentim& Andrade, Tadeu Uggere& Endringer, Denise Coutinho…[et al.]. Automated and Reproducible Detection of Vascular Endothelial Growth Factor (VEGF) in Renal Tissue Sections. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1180705
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1180705