Genetic Manipulation of Calcium Release-Activated Calcium Channel 1 Modulates the Multipotency of Human Cartilage-Derived Mesenchymal Stem Cells
Joint Authors
Mogi, Masaki
Liu, Shuang
Takahashi, Minae
Kiyoi, Takeshi
Toyama, Kensuke
Source
Journal of Immunology Research
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Calcium is a ubiquitous intracellular messenger that has a crucial role in determining the proliferation, differentiation, and functions of multipotent mesenchymal stem cells (MSCs).
Our study is aimed at elucidating the influence of genetically manipulating Ca2+ release-activated Ca2+ (CRAC) channel-mediated intercellular Ca2+ signaling on the multipotency of MSCs.
The abilities of genetically engineered MSCs, including CRAC-overexpressing and CRAC-knockout MSCs, to differentiate into multiple mesenchymal lineages, including adipogenic, osteogenic, and chondrogenic lineages, were evaluated.
CRAC channel-mediated Ca2+ influx into these cells was regulated, and the differentiation fate of MSCs was modified.
Upregulation of intracellular Ca2+ signals attenuated the adipogenic differentiation ability and slightly increased the osteogenic differentiation potency of MSCs, whereas downregulation of CRACM1 expression promoted chondrogenic differentiation potency.
The findings demonstrated the effects of genetically manipulating MSCs by targeting CRACM1.
CRAC-modified MSCs had distinct differentiation fates to adipocytes, osteoblasts, and chondrocytes.
To aid in the clinical implementation of tissue engineering strategies for joint regeneration, these data may allow us to identify prospective factors for effective treatments and could maximize the therapeutic potential of MSC-based transplantation.
American Psychological Association (APA)
Liu, Shuang& Takahashi, Minae& Kiyoi, Takeshi& Toyama, Kensuke& Mogi, Masaki. 2019. Genetic Manipulation of Calcium Release-Activated Calcium Channel 1 Modulates the Multipotency of Human Cartilage-Derived Mesenchymal Stem Cells. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1180808
Modern Language Association (MLA)
Liu, Shuang…[et al.]. Genetic Manipulation of Calcium Release-Activated Calcium Channel 1 Modulates the Multipotency of Human Cartilage-Derived Mesenchymal Stem Cells. Journal of Immunology Research No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1180808
American Medical Association (AMA)
Liu, Shuang& Takahashi, Minae& Kiyoi, Takeshi& Toyama, Kensuke& Mogi, Masaki. Genetic Manipulation of Calcium Release-Activated Calcium Channel 1 Modulates the Multipotency of Human Cartilage-Derived Mesenchymal Stem Cells. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1180808
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1180808