Quantitative Analysis of Intracellular Motility Based on Optical Flow Model
Joint Authors
Huang, Yali
Liu, Zhiwen
Hao, Lei
Li, Heng
Wang, Pei-Guang
Source
Journal of Healthcare Engineering
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Analysis of cell mobility is a key issue for abnormality identification and classification in cell biology research.
However, since cell deformation induced by various biological processes is random and cell protrusion is irregular, it is difficult to measure cell morphology and motility in microscopic images.
To address this dilemma, we propose an improved variation optical flow model for quantitative analysis of intracellular motility, which not only extracts intracellular motion fields effectively but also deals with optical flow computation problem at the border by taking advantages of the formulation based on L1 and L2 norm, respectively.
In the energy functional of our proposed optical flow model, the data term is in the form of L2 norm; the smoothness of the data changes with regional features through an adaptive parameter, using L1 norm near the edge of the cell and L2 norm away from the edge.
We further extract histograms of oriented optical flow (HOOF) after optical flow field of intracellular motion is computed.
Then distances of different HOOFs are calculated as the intracellular motion features to grade the intracellular motion.
Experimental results show that the features extracted from HOOFs provide new insights into the relationship between the cell motility and the special pathological conditions.
American Psychological Association (APA)
Huang, Yali& Hao, Lei& Li, Heng& Liu, Zhiwen& Wang, Pei-Guang. 2017. Quantitative Analysis of Intracellular Motility Based on Optical Flow Model. Journal of Healthcare Engineering،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1180822
Modern Language Association (MLA)
Huang, Yali…[et al.]. Quantitative Analysis of Intracellular Motility Based on Optical Flow Model. Journal of Healthcare Engineering No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1180822
American Medical Association (AMA)
Huang, Yali& Hao, Lei& Li, Heng& Liu, Zhiwen& Wang, Pei-Guang. Quantitative Analysis of Intracellular Motility Based on Optical Flow Model. Journal of Healthcare Engineering. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1180822
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1180822