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Human Hepatocytes and Differentiated Adult-Derived Human Liver StemProgenitor Cells Display In Vitro Immunosuppressive Properties Mediated, at Least in Part, through the Nonclassical HLA Class I Molecule HLA-G
Joint Authors
Najimi, Mustapha
Carosella, Edgardo D.
LeMaoult, Joel
Sokal, Etienne
Ravau, Joachim
André, Floriane
Lombard, Catherine
Sana, Gwenaëlle
Najar, Mehdi
Bouhtit, Fatima
Daouya, Marina
Loustau, Maria
Lagneaux, Laurence
Source
Journal of Immunology Research
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-09-12
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
One of the main challenges in liver cell therapy (LCT) is the induction of a tolerogenic microenvironment to promote graft acceptance in the recipient.
Little is known about the immunomodulatory potential of the hepatic cells used in liver cell therapy.
In this work, we wanted to evaluate the immunosuppressive properties of human hepatocytes and adult-derived human liver stem/progenitor cells (ADHLSCs), as well as the potential involvement of the immunomodulatory molecule HLA-G.
We demonstrated that both cell types were capable of inhibiting the proliferative response of PBMCs to an allogenic stimulus and that the immune inhibitory potential of ADHLSCs, although lower than that of hepatocytes, increased after hepatogenic differentiation.
We demonstrated that liver cells express HLA-G and that the immune inhibition pattern was clearly associated to its expression.
Interestingly, HLA-G expression increased after the third step of differentiation, wherein oncostatin M (OSM) was added.
A 48 hr treatment with OSM was sufficient to induce HLA-G expression in ADHLSCs and result in immune inhibition.
Surprisingly, blocking HLA-G partially reversed the immune inhibition mediated by hepatocytes and differentiated ADHLSCs, but not that of undifferentiated ADHLSCs, suggesting that additional immune inhibitory mechanisms may be used by these cells.
In conclusion, we demonstrated that both hepatocytes and ADHLSCs present immunomodulatory properties mediated, at least in part, through HLA-G, which can be upregulated following hepatogenic differentiation or liver cell pretreatment with OSM.
These observations open up new perspectives for the induction of tolerance following LCT and for potential therapeutic applications of these liver cells.
American Psychological Association (APA)
Lombard, Catherine& Sana, Gwenaëlle& LeMaoult, Joel& Najar, Mehdi& Ravau, Joachim& André, Floriane…[et al.]. 2019. Human Hepatocytes and Differentiated Adult-Derived Human Liver StemProgenitor Cells Display In Vitro Immunosuppressive Properties Mediated, at Least in Part, through the Nonclassical HLA Class I Molecule HLA-G. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1180916
Modern Language Association (MLA)
Lombard, Catherine…[et al.]. Human Hepatocytes and Differentiated Adult-Derived Human Liver StemProgenitor Cells Display In Vitro Immunosuppressive Properties Mediated, at Least in Part, through the Nonclassical HLA Class I Molecule HLA-G. Journal of Immunology Research No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1180916
American Medical Association (AMA)
Lombard, Catherine& Sana, Gwenaëlle& LeMaoult, Joel& Najar, Mehdi& Ravau, Joachim& André, Floriane…[et al.]. Human Hepatocytes and Differentiated Adult-Derived Human Liver StemProgenitor Cells Display In Vitro Immunosuppressive Properties Mediated, at Least in Part, through the Nonclassical HLA Class I Molecule HLA-G. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1180916
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1180916