Bone Marrow Mesenchymal Stem Cells Decrease the Expression of RANKL in Collagen-Induced Arthritis Rats via Reducing the Levels of IL-22
Joint Authors
Li, Xiaofeng
Gao, Chong
Li, Fang
Li, Xin
Liu, Guiyan
Source
Journal of Immunology Research
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
To investigate the transplantation effect of bone marrow mesenchymal stem cells (MSCs) on the expression of interlukin-22 (IL-22) and RANKL in collagen-induced arthritis (CIA) rats.
Methods.
32 CIA models were established.
16 CIA rats were transplanted with MSCs, and others were used as nontreatment CIA controls.
The concentrations of IL-22 and RANKL in serum were detected by ELISA and those in synovial tissue of rats’ joints by immunohistochemical staining.
In addition, the expression of RANKL mRNA was measured by RT-PCR in the fibroblast-like synoviocytes (FLSs), cultured with IL-22 in vitro, which were delivered from the joints of CIA rats treated with or without MSCs.
Results.
The transplantation of MSCs into CIA rats relieved the destruction of joints, measured by AI score, X-ray, and histopathology.
MSCs also reduced the expression of IL-22 and RANKL in serum by ELISA (P<0.001) and similarly in FLSs by immunohistochemical staining.
In vitro, IL-22 induced significantly the expression of RANKL mRNA in cultured FLSs in a dose-dependent manner, whereas this induction was significantly reduced in FLSs derived from CIA rats transplanted with MSCs (normal controls: F=79.33, P<0.001; CIA controls: F=712.72, P<0.001; and CIA-MSC rats: F=139.04, P<0.001).
Conclusion.
Our results suggest that the transplantation of MSCs can reduce the expression of RANKL in vivo by downregulating the levels of IL-22, thereby ameliorating the degree of RA bone destruction.
This study provides a theoretical basis for a potential therapy of RA with MSCs, and IL-22 and RANKL may become two new targets to treat RA.
American Psychological Association (APA)
Li, Fang& Li, Xin& Liu, Guiyan& Gao, Chong& Li, Xiaofeng. 2019. Bone Marrow Mesenchymal Stem Cells Decrease the Expression of RANKL in Collagen-Induced Arthritis Rats via Reducing the Levels of IL-22. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1180951
Modern Language Association (MLA)
Li, Fang…[et al.]. Bone Marrow Mesenchymal Stem Cells Decrease the Expression of RANKL in Collagen-Induced Arthritis Rats via Reducing the Levels of IL-22. Journal of Immunology Research No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1180951
American Medical Association (AMA)
Li, Fang& Li, Xin& Liu, Guiyan& Gao, Chong& Li, Xiaofeng. Bone Marrow Mesenchymal Stem Cells Decrease the Expression of RANKL in Collagen-Induced Arthritis Rats via Reducing the Levels of IL-22. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1180951
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1180951