Sildenafil Citrate Influences Production of TNF-α in Healthy Men Lymphocytes
Joint Authors
Zagozdzon, Radosław
Zych, Michał
Roszczyk, Aleksander
Kniotek, Monika
Kaleta, Beata
Source
Journal of Immunology Research
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-10-22
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The aim of our study was to determine whether sildenafil citrate influences the production of Th1- (TNF-α, INF-γ) or Th2-type (TGF-β, IL-10) cytokines by lymphocytes of healthy men.
Sildenafil citrate (SC) is a selective blocker of phosphodiesterase 5, by competing for the binding site with cGMP.
It was reported that a higher risk of sexually transmitted diseases (STD) could be correlated with a recreational use of sildenafil, especially when combined with another drug.
While behavioral causes of these findings are understood, it is worth considering other causes of that phenomenon that might rely on the influence of sildenafil on the immune system.
Material and Methods.
Peripheral blood mononuclear cells (PBMCs) were isolated from 27 healthy men donors and cultured in the presence of SC at a concentration of 400 ng/ml.
The first set of research was performed on cells stimulated, for at least 4 hours, by incubation with phorbol myristate acetate (PMA), ionomycin, and Golgi-Stop.
Subsequently, we determined cytokine production in cells stimulated with phytohemagglutinin (PHA) for 12 hours in the presence of Golgi-Stop.
Flow cytometry immunophenotyping of PBMC was performed towards the surface marker of T cells: CD3 and intracellular cytokine expression: TNF-α, IFN-γ, TGF-β, and IL-10.
Our findings show that SC significantly decreased the percentage of T cells producing TNF-α and displayed tendency to decrease IFN-γ, when stimulated with PMA.
Frequent usage of SC might strengthen this effect.
That could partially explain the impaired immune response to the pathogens of men using the drug.
American Psychological Association (APA)
Zych, Michał& Roszczyk, Aleksander& Kniotek, Monika& Kaleta, Beata& Zagozdzon, Radosław. 2019. Sildenafil Citrate Influences Production of TNF-α in Healthy Men Lymphocytes. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1180954
Modern Language Association (MLA)
Zych, Michał…[et al.]. Sildenafil Citrate Influences Production of TNF-α in Healthy Men Lymphocytes. Journal of Immunology Research No. 2019 (2019), pp.1-6.
https://search.emarefa.net/detail/BIM-1180954
American Medical Association (AMA)
Zych, Michał& Roszczyk, Aleksander& Kniotek, Monika& Kaleta, Beata& Zagozdzon, Radosław. Sildenafil Citrate Influences Production of TNF-α in Healthy Men Lymphocytes. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1180954
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1180954