Ovarian Cancer Stem Cells with High ROR1 Expression Serve as a New Prophylactic Vaccine for Ovarian Cancer
Joint Authors
Wu, Di
Yu, Xiaoyu
Wang, Jing
Hui, Xu
Zhang, Yunxia
Cai, Yunlang
Ren, Mulan
Guo, Mei
Zhao, Fengshu
Dou, Jun
Source
Journal of Immunology Research
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-17
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Tumor vaccines offer a number of advantages for cancer treatment.
In the study, the vaccination with cancer stem cells (CSCs) with high expression of the type I receptor tyrosine kinase-like orphan receptor (ROR1) was evaluated in a murine model for the vaccine’s immunogenicity and protective efficacy against epithelial ovarian carcinoma (EOC).
CD117+CD44+ CSCs were isolated from human EOC HO8910 cell line using a magnetic-activated cell sorting system; murine ID8 EOC suspension sphere cells, which are collectively known as cancer stem-like cells, were acquired from serum-free suspension sphere-forming culture.
Mice were subcutaneously immunized with the repeat cycles of freezing and thawing whole HO8910 CD117+CD44+ CSCs and ID8 cancer stem-like cells, respectively, followed by a challenge with HO8910 or ID8 cells at one week after final vaccination.
The results showed that the CSC vaccination significantly induced immunity against EOC growth and markedly prolonged the survival of EOC-bearing mice in the prophylactic setting compared with non-CSC vaccination.
Flow cytometry showed significantly increased immunocyte cytotoxicities and remarkably reduced CSC counts in the CSC-vaccinated mice.
Moreover, the protective efficacy against EOC was decreased when the ROR1 expression was downregulated by shRNA in CSC vaccines.
The findings from the study suggest that CSC vaccines with high ROR1 expression were highly effective in triggering immunity against EOC in vaccinated mice and may serve as an effective vaccine for EOC immunoprophylaxis.
American Psychological Association (APA)
Wu, Di& Yu, Xiaoyu& Wang, Jing& Hui, Xu& Zhang, Yunxia& Cai, Yunlang…[et al.]. 2019. Ovarian Cancer Stem Cells with High ROR1 Expression Serve as a New Prophylactic Vaccine for Ovarian Cancer. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1181067
Modern Language Association (MLA)
Wu, Di…[et al.]. Ovarian Cancer Stem Cells with High ROR1 Expression Serve as a New Prophylactic Vaccine for Ovarian Cancer. Journal of Immunology Research No. 2019 (2019), pp.1-16.
https://search.emarefa.net/detail/BIM-1181067
American Medical Association (AMA)
Wu, Di& Yu, Xiaoyu& Wang, Jing& Hui, Xu& Zhang, Yunxia& Cai, Yunlang…[et al.]. Ovarian Cancer Stem Cells with High ROR1 Expression Serve as a New Prophylactic Vaccine for Ovarian Cancer. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1181067
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1181067