Reverse Immunology Approach to Define a New HIV-gp41-Neutralizing Epitope
Joint Authors
Dorgham, Karim
Pietrancosta, Nicolas
Affoune, Amel
Lucar, Olivier
Bouceba, Tahar
Chardonnet, Solenne
Pionneau, Cedric
Piesse, Christophe
Sterlin, Delphine
Guardado-Calvo, Pablo
Karoyan, Philippe
Debré, Patrice
Gorochov, Guy
Vieillard, Vincent
Source
Journal of Immunology Research
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-24
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The design of immunogens susceptible to elicit potent and broadly neutralizing antibodies against the human immunodeficiency virus type 1 (HIV-1) remains a veritable challenge in the course of vaccine development.
Viral envelope proteins adopt different conformational states during the entry process, allowing the presentation of transient neutralizing epitopes.
We focused on the highly conserved 3S motif of gp41, which is exposed to the surface envelope in its trimeric prefusion state.
Vaccination with a W614A-modified 3S peptide induces in animals neutralizing anti-HIV-1 antibodies among which we selected clone F8.
We used F8 as bait to select for W614A-3S phage-peptide mimics.
Binding and molecular docking studies revealed that F8 interacts similarly with W614A-3S and a Mim_F8-1 mimotope, despite their lack of sequence homology, suggesting structural mimicry.
Finally, vaccination of mice with the purified Mim_F8-1 phage elicited HIV-1-neutralizing antibodies that bound to the cognate W614A-3S motif.
Collectively, our findings provide new insights into the molecular design of immunogens to elicit antibodies with neutralizing properties.
American Psychological Association (APA)
Dorgham, Karim& Pietrancosta, Nicolas& Affoune, Amel& Lucar, Olivier& Bouceba, Tahar& Chardonnet, Solenne…[et al.]. 2019. Reverse Immunology Approach to Define a New HIV-gp41-Neutralizing Epitope. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1181169
Modern Language Association (MLA)
Dorgham, Karim…[et al.]. Reverse Immunology Approach to Define a New HIV-gp41-Neutralizing Epitope. Journal of Immunology Research No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1181169
American Medical Association (AMA)
Dorgham, Karim& Pietrancosta, Nicolas& Affoune, Amel& Lucar, Olivier& Bouceba, Tahar& Chardonnet, Solenne…[et al.]. Reverse Immunology Approach to Define a New HIV-gp41-Neutralizing Epitope. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1181169
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1181169