Scavenger Receptor Class B Member 1 Independent Uptake of Transthyretin by Cultured Hepatocytes Is Regulated by High Density Lipoprotein
Joint Authors
d’Emden, Michael C.
Richard, Kerry
Landers, Kelly A.
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Thyroid hormone (thyroxine, T4) is essential for the normal function of all cell types and is carried in serum bound to several proteins including transthyretin.
Recently, evidence has emerged of alternate pathways for hormone entry into cells that are dependent on hormone binding proteins.
Transthyretin and transthyretin bound T4 are endocytosed by placental trophoblasts through the high-density lipoprotein receptor, Scavenger Receptor Class B Type 1 (SR-B1).
High density lipoprotein (HDL) affects the expression and function of SR-B1 in trophoblast cells.
SR-B1 is also expressed in hepatocytes and we sought to determine if hepatocyte SR-B1 was involved in transthyretin or transthyretin-T4 uptake and whether uptake was affected by HDL.
Transthyretin and transthyretin-T4 uptake by hepatocytes is not dependent on SR-B1.
HDL treatment reduced SR-B1 expression.
However, pretreatment of hepatocytes with HDL increased uptake of transthyretin-T4.
Knockdown of SR-B1 expression using siRNA also increased transthyretin-T4 uptake.
Coaddition of HDL to transthyretin uptake experiments blocked both transthyretin and transthyretin-T4 uptake.
Hepatocyte uptake of transthyretin-T4 uptake is influenced by, but is not dependent on, SR-B1 expression.
HDL also decreases transthyretin-T4 uptake and therefore diet or drugs may interfere with this process.
This suggests that multiple lipoprotein receptors may be involved in the regulation of uptake of transthyretin-T4 in a cell-type specific manner.
Further study is required to understand this important process.
American Psychological Association (APA)
Landers, Kelly A.& d’Emden, Michael C.& Richard, Kerry. 2019. Scavenger Receptor Class B Member 1 Independent Uptake of Transthyretin by Cultured Hepatocytes Is Regulated by High Density Lipoprotein. Journal of Lipids،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1181330
Modern Language Association (MLA)
Landers, Kelly A.…[et al.]. Scavenger Receptor Class B Member 1 Independent Uptake of Transthyretin by Cultured Hepatocytes Is Regulated by High Density Lipoprotein. Journal of Lipids No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1181330
American Medical Association (AMA)
Landers, Kelly A.& d’Emden, Michael C.& Richard, Kerry. Scavenger Receptor Class B Member 1 Independent Uptake of Transthyretin by Cultured Hepatocytes Is Regulated by High Density Lipoprotein. Journal of Lipids. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1181330
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1181330