Oxidative Stress and Apoptosis Contributed to Nonylphenol-Induced Cell Damage in Mouse NCTC Clone 1469 Cells
Joint Authors
Liu, Xiaozhen
Li, Fuxiang
Chen, Yangjie
Zhu, Zhaoliang
Peng, Gaoyi
Xie, Mingyong
Nie, Shaoping
Yu, Qiang
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-26
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Nonylphenol (NP) is considered an environmental toxicant and endocrine-disrupting compound.
The present study aimed to investigate the effects of NP on NCTC Clone 1469, nonparenchymal hepatocytes, and to study the molecular basis of NP-induced liver injury.
The results showed that NP decreased cell viability and induced nucleus crenulation and intracellular enzyme leakage in NCTC Clone 1469 cells.
Additionally, NP-induced oxidative stress and apoptosis of NCTC Clone 1469 are accompanied by upregulating reactive oxygen species (ROS) production, increase of Bax, decrease of Bcl-2, activation of caspase-3 and caspase-12, and release of cytosolic free Ca2+ in the cells.
ROS scavenger, N-acetyl-L-cysteine (NAC), prevented the intracellular enzyme leakage induced by NP.
NP induced alteration of estrogen receptor- (ER-) α and ER-β expression, while ER antagonists, ICI 182,780, showed no effect on NP-induced intracellular enzyme leakage.
We proposed that NP triggered cell damage via inducing oxidative stress and apoptosis in cells, but not estrogenic effect.
American Psychological Association (APA)
Liu, Xiaozhen& Chen, Yangjie& Nie, Shaoping& Li, Fuxiang& Zhu, Zhaoliang& Peng, Gaoyi…[et al.]. 2020. Oxidative Stress and Apoptosis Contributed to Nonylphenol-Induced Cell Damage in Mouse NCTC Clone 1469 Cells. Journal of Chemistry،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1181349
Modern Language Association (MLA)
Liu, Xiaozhen…[et al.]. Oxidative Stress and Apoptosis Contributed to Nonylphenol-Induced Cell Damage in Mouse NCTC Clone 1469 Cells. Journal of Chemistry No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1181349
American Medical Association (AMA)
Liu, Xiaozhen& Chen, Yangjie& Nie, Shaoping& Li, Fuxiang& Zhu, Zhaoliang& Peng, Gaoyi…[et al.]. Oxidative Stress and Apoptosis Contributed to Nonylphenol-Induced Cell Damage in Mouse NCTC Clone 1469 Cells. Journal of Chemistry. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1181349
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1181349