Application of Nanoscale Zwitterionic Polyelectrolytes Brush with High Stability and Quantum Yield in Aqueous Solution for Cell Imaging
Joint Authors
Fan, Quli
Lu, Xiaomei
Zhang, Zhiyong
Tan, Minqiang
Kong, Linghui
Sun, Pengfei
Mo, Hua
Huang, Wei
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-27
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Cationic and zwitterionic polyelectrolytes are synthesized through atom transfer radical polymerization (ATRP), comprising a polyfluorene backbone with a small fraction of 2,1,3-benzothiadiazole and poly[2-(dimethylamino)ethyl methacrylate] (PDMAEMA) side chains.
Due to higher charge density generated from grafted side chains, two polymers show higher water solubility and higher quantum yield.
In comparison with cationic polyelectrolytes, zwitterionic polyelectrolytes are stable over a broad pH range from 1 to 13, even in 1 M NaCl solution.
The absence of FRET between zwitterionic polymers and dye-labeled ssDNA indicates their ultralow nonspecific adsorption, while cationic polymer shows much stronger nonspecific interactions.
The MTT assay of zwitterionic polymers exhibits their minimal cytotoxicity and potential in long-term clinical application.
Most importantly, zwitterionic polymer could be efficiently taken up by cells, whereas cationic polymer stains the surface of cell due to membrane disruption generated from positive charges.
The results illustrate that conjugated zwitterionic polymer could serve as a novel type of highly efficient ultralow fouling material with low cytotoxicity for labelling cell or potential biomedical applications.
American Psychological Association (APA)
Zhang, Zhiyong& Tan, Minqiang& Kong, Linghui& Lu, Xiaomei& Sun, Pengfei& Mo, Hua…[et al.]. 2020. Application of Nanoscale Zwitterionic Polyelectrolytes Brush with High Stability and Quantum Yield in Aqueous Solution for Cell Imaging. Journal of Chemistry،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1181452
Modern Language Association (MLA)
Zhang, Zhiyong…[et al.]. Application of Nanoscale Zwitterionic Polyelectrolytes Brush with High Stability and Quantum Yield in Aqueous Solution for Cell Imaging. Journal of Chemistry No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1181452
American Medical Association (AMA)
Zhang, Zhiyong& Tan, Minqiang& Kong, Linghui& Lu, Xiaomei& Sun, Pengfei& Mo, Hua…[et al.]. Application of Nanoscale Zwitterionic Polyelectrolytes Brush with High Stability and Quantum Yield in Aqueous Solution for Cell Imaging. Journal of Chemistry. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1181452
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1181452