Genetic Clearness Novel Strategy of Group I Bacillus Species Isolated from Fermented Food and Beverages by Using Fibrinolytic Enzyme Gene Encoding a Serine-Like Enzyme
Joint Authors
Kayath, Christian Aimé
Lebonguy, Aimé Augustin
Nguimbi, Etienne
Kaya-Ongoto, Moïse Doria
Nzaou, Stech Anomène Eckzechel
Elenga Wilson, Paola Sandra
Ahombo, Gabriel
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-20
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Fibrinolytic enzyme gene (fibE) is widely conserved among Bacillus spp.
belonging to group I species.
This is encoding a serine-like enzyme (FibE) secreted in extracellular medium.
This present work aims to assess the molecular usefulness of this novel conserved housekeeping gene among group I Bacillus spp.
to identify and discriminate some related strains in traditional fermented food and beverages in Republic of Congo.
First of all 155 isolates have been screened for enzymatic activities using caseinolytic assays.
PCR techniques and nested PCR method using specific primers and correlated with 16S RNA sequencing were used.
Blotting techniques have been performed for deep comparison with molecular methods.
As a result B.
amyloliquefaciens (1), B.
licheniformis (1), B.
subtilis (1), B.
pumilus (3), B.
altitudinis (2), B.
atrophaeus (1), and B.
safensis (3) have been specifically identified among 155 isolates found in fermented food and beverages.
Genetic analysis and overexpression of glutathione S-transferases (GSTs) fused to mature protein of FibE in Escherichia coli BL21 and TOP10 showed 2-fold higher enzymatic activities by comparison with FibE wild type one.
Immunodetection should be associated but this does not clearly discriminate Bacillus belonging to group I.
American Psychological Association (APA)
Kaya-Ongoto, Moïse Doria& Kayath, Christian Aimé& Nguimbi, Etienne& Lebonguy, Aimé Augustin& Nzaou, Stech Anomène Eckzechel& Elenga Wilson, Paola Sandra…[et al.]. 2019. Genetic Clearness Novel Strategy of Group I Bacillus Species Isolated from Fermented Food and Beverages by Using Fibrinolytic Enzyme Gene Encoding a Serine-Like Enzyme. Journal of Nucleic Acids،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1181488
Modern Language Association (MLA)
Kaya-Ongoto, Moïse Doria…[et al.]. Genetic Clearness Novel Strategy of Group I Bacillus Species Isolated from Fermented Food and Beverages by Using Fibrinolytic Enzyme Gene Encoding a Serine-Like Enzyme. Journal of Nucleic Acids No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1181488
American Medical Association (AMA)
Kaya-Ongoto, Moïse Doria& Kayath, Christian Aimé& Nguimbi, Etienne& Lebonguy, Aimé Augustin& Nzaou, Stech Anomène Eckzechel& Elenga Wilson, Paola Sandra…[et al.]. Genetic Clearness Novel Strategy of Group I Bacillus Species Isolated from Fermented Food and Beverages by Using Fibrinolytic Enzyme Gene Encoding a Serine-Like Enzyme. Journal of Nucleic Acids. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1181488
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1181488