A Novel System for the Quantification of the ADCC Activity of Therapeutic Antibodies
Joint Authors
Lallemand, Christophe
Liang, Feifei
Staub, Flore
Simansour, Maud
Vallette, Benoit
Huang, Lue
Ferrando-Miguel, Rosa
Tovey, Michael G.
Source
Journal of Immunology Research
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-27
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Abstract EN
Novel ADCC effector cells expressing the V-variant or F-variant of FcγRIIIa (CD16a) and firefly luciferase under the control of a chimeric promoter incorporating recognition sequences for the principal transcription factors involved in FcγRIIIa signal transduction, together with novel target cells overexpressing a constant high level of the specific antigen recognized by rituximab, trastuzumab, cetuximab, infliximab, adalimumab, or etanercept, confer improved sensitivity, specificity, and dynamic range in an ADCC assay relative to effector cells expressing a NFAT-regulated reporter gene and wild-type target cells.
The effector cells also contain a normalization gene rendering ADCC assays independent of cell number or serum matrix effects.
The novel effector and target cells in a frozen thaw-and-use format exhibit low vial-to-vial and lot-to-lot variation in their performance characteristics reflected by CVs of 10% or less.
Homologous control target cells in which the specific target gene has been invalidated by genome editing providing an ideal control and a means of correcting for nonspecific effects were observed with certain samples of human serum.
The novel effector cells and target cells expressing noncleavable membrane-bound TNFα have been used to quantify ADCC activity in serum from patients with Crohn’s disease treated with infliximab and to relate ADCC activity to drug levels.
American Psychological Association (APA)
Lallemand, Christophe& Liang, Feifei& Staub, Flore& Simansour, Maud& Vallette, Benoit& Huang, Lue…[et al.]. 2017. A Novel System for the Quantification of the ADCC Activity of Therapeutic Antibodies. Journal of Immunology Research،Vol. 2017, no. 2017, pp.1-19.
https://search.emarefa.net/detail/BIM-1181754
Modern Language Association (MLA)
Lallemand, Christophe…[et al.]. A Novel System for the Quantification of the ADCC Activity of Therapeutic Antibodies. Journal of Immunology Research No. 2017 (2017), pp.1-19.
https://search.emarefa.net/detail/BIM-1181754
American Medical Association (AMA)
Lallemand, Christophe& Liang, Feifei& Staub, Flore& Simansour, Maud& Vallette, Benoit& Huang, Lue…[et al.]. A Novel System for the Quantification of the ADCC Activity of Therapeutic Antibodies. Journal of Immunology Research. 2017. Vol. 2017, no. 2017, pp.1-19.
https://search.emarefa.net/detail/BIM-1181754
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1181754