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Immobilization of Protein A on Monodisperse Magnetic Nanoparticles for Biomedical Applications
Joint Authors
Tran, Ngoc Quyen
Nguyen, Tan Tai
Bashir, Mohammed J. K.
Ngo, Quang Minh
Phan, Thang Bach
Thanh, Bui Trung
Van Sau, Nguyen
Ju, Heongkyu
Hai, Tran Hoang
Van, Pham Hung
Jun, H. K.
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
We presented synthesis and physical characterization of iron oxide magnetic nanoparticles (Fe3O4) for biomedical applications in the size range of 10-30 nm.
Magnetic nanoparticles were synthesized by the coprecipitation method, and the particles’ size was controlled by two different injection methods of sodium hydroxide (NaOH).
The synthesized magnetic nanoparticles were then modified by using series of linkers including tetraethyl orthosilicate (TEOS), 3-aminopropyltriethoxysilane (APTES), and glutaraldehyde (GA) to generate the structure of Fe3O4/SiO2/NH2/CHO, which can be used for immobilization of protein A.
Additionally, we used transmission electron microscopy (TEM), X-ray powder diffraction (XRD), vibrating-sample magnetometry (VSM), and Fourier-transform infrared spectroscopy (FTIR), for characterization of properties and structure of the nanoparticles.
An immobilization of protein A on magnetic nanoparticles was studied with a UV-Vis spectrum (UV-Vis) and fluorescence electron microscopy and Bradford method.
Results showed that an XRD spectrum with a peak at (311) corresponded to the standard peak of magnetic nanoparticles.
In addition, the magnetic nanoparticles with d≥30 nm have higher saturation magnetizations in comparison with the smaller ones with d≤10 nm.
However, the smaller magnetic nanoparticles offered higher efficiency for binding of protein A, due to the high surface/volume ratio.
These particles with functional groups on their surface are promising candidates for biomedical applications, e.g., drug delivery, controlled drug release, or disease diagnosis in point-of-care test.
American Psychological Association (APA)
Thanh, Bui Trung& Van Sau, Nguyen& Ju, Heongkyu& Bashir, Mohammed J. K.& Jun, H. K.& Phan, Thang Bach…[et al.]. 2019. Immobilization of Protein A on Monodisperse Magnetic Nanoparticles for Biomedical Applications. Journal of Nanomaterials،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1181776
Modern Language Association (MLA)
Thanh, Bui Trung…[et al.]. Immobilization of Protein A on Monodisperse Magnetic Nanoparticles for Biomedical Applications. Journal of Nanomaterials No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1181776
American Medical Association (AMA)
Thanh, Bui Trung& Van Sau, Nguyen& Ju, Heongkyu& Bashir, Mohammed J. K.& Jun, H. K.& Phan, Thang Bach…[et al.]. Immobilization of Protein A on Monodisperse Magnetic Nanoparticles for Biomedical Applications. Journal of Nanomaterials. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1181776
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1181776