Decitabine-Induced Changes in Human Myelodysplastic Syndrome Cell Line SKM-1 Are Mediated by FOXO3A Activation
Joint Authors
Zeng, Wen
Dai, Hanjun
Yan, Ming
Cai, Xiaojun
Luo, Hong
Ke, Min
Liu, Zeming
Source
Journal of Immunology Research
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-16
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The epigenetic silencing of tumor suppressor genes in myelodysplastic syndromes (MDS) can potentially confer a growth advantage to individual cellular clones.
Currently, the recommended treatment for patients with high-risk MDS is the methylation agent decitabine (DAC), a drug that can induce the reexpression of silenced tumor suppressor genes.
We investigated the effects of DAC treatment on the myeloid MDS cell line SKM-1 and investigated the role of FOXO3A, a potentially tumor-suppressive transcription factor, by silencing its expression prior to DAC treatment.
We found that FOXO3A exists in an inactive, hyperphosphorylated form in SKM-1 cells, but that DAC both induces FOXO3A expression and reactivates the protein by reducing its phosphorylation level.
Furthermore, we show that this FOXO3A activation is responsible for the DAC-induced differentiation of SKM-1 cells into monocytes, as well as for SKM-1 cell cycle arrest, apoptosis, and autophagy.
Collectively, these results suggest that FOXO3A reactivation may contribute to the therapeutic effects of DAC in MDS.
American Psychological Association (APA)
Zeng, Wen& Dai, Hanjun& Yan, Ming& Cai, Xiaojun& Luo, Hong& Ke, Min…[et al.]. 2017. Decitabine-Induced Changes in Human Myelodysplastic Syndrome Cell Line SKM-1 Are Mediated by FOXO3A Activation. Journal of Immunology Research،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1181785
Modern Language Association (MLA)
Zeng, Wen…[et al.]. Decitabine-Induced Changes in Human Myelodysplastic Syndrome Cell Line SKM-1 Are Mediated by FOXO3A Activation. Journal of Immunology Research No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1181785
American Medical Association (AMA)
Zeng, Wen& Dai, Hanjun& Yan, Ming& Cai, Xiaojun& Luo, Hong& Ke, Min…[et al.]. Decitabine-Induced Changes in Human Myelodysplastic Syndrome Cell Line SKM-1 Are Mediated by FOXO3A Activation. Journal of Immunology Research. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1181785
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1181785