![](/images/graphics-bg.png)
An Exploration of the Impact of Anticentromere Antibody on Early-Stage Embryo
Joint Authors
Ying, Ying
Guo, Xi
Zhong, Yiping
Zhou, Can-Quan
Source
Journal of Immunology Research
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-04
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Background.
Previously, we found women with positive anticentromere antibody showed impaired potential of oocyte maturation and embryo cleavage; the possible mechanism behind this phenomenon was still unknown.
Objective.
Thus, the present study aimed to preliminarily explore whether ACA could penetrate into the living embryos and impair their developmental potential via in vitro coculture with mouse embryos.
Methods.
Mouse embryos were collected and used for in vitro culture with polyclonal anticentromere protein A (CENP-A) antibody; then, immunofluorescence assay was performed to determine the penetration of antibody into embryos, and embryo development potential was observed.
Results.
All embryos cultured with anti-CENP-A antibody exhibited immunofluorescence on the nucleus, while none of the embryos from the control groups showed immunofluorescence.
Additionally, embryos cultured with anti-CENP-A antibody experienced significant growth impairment compared with controls.
Conclusion.
Mouse embryos may be a direct target for ACA in vitro prior to implantation.
However, the precise mechanism needs further clarification.
American Psychological Association (APA)
Ying, Ying& Guo, Xi& Zhong, Yiping& Zhou, Can-Quan. 2017. An Exploration of the Impact of Anticentromere Antibody on Early-Stage Embryo. Journal of Immunology Research،Vol. 2017, no. 2017, pp.1-4.
https://search.emarefa.net/detail/BIM-1181868
Modern Language Association (MLA)
Ying, Ying…[et al.]. An Exploration of the Impact of Anticentromere Antibody on Early-Stage Embryo. Journal of Immunology Research No. 2017 (2017), pp.1-4.
https://search.emarefa.net/detail/BIM-1181868
American Medical Association (AMA)
Ying, Ying& Guo, Xi& Zhong, Yiping& Zhou, Can-Quan. An Exploration of the Impact of Anticentromere Antibody on Early-Stage Embryo. Journal of Immunology Research. 2017. Vol. 2017, no. 2017, pp.1-4.
https://search.emarefa.net/detail/BIM-1181868
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1181868