Characterization of Phenolic Constituents from Prunus cerasifera Ldb Leaves

Abstract EN

To elucidate the chemical compositions of Prunus cerasifera Ldb leaves, the methanol extracts were firstly fractionated by ethyl acetate and n-butanol, respectively.

The phenolic acid-rich fractions (ethyl acetate extracts) were further isolated by various chromatographic columns (CC) including MCI macroporous resin, normal-phase silica gel, Sephadex gel LH-20, octadecyl silane (ODS), and preparative HPLC to yield the phenolic compounds.

The isolated compounds were analyzed by 1H-nuclear magnetic resonance (1H-NMR), 13C-NMR, and electrospray ionization mass spectral (ESI-MS) spectroscopy.

Eleven phenolic acids were identified as p-coumaric acid (1), caffeic acid (2), ferulic acid (3), chlorogenic acid (4), 3-O-caffeoylquinic acid (5), 5-O-coumaroylquinic acid (6), 3-O-caffeoylquinic acid methyl ester (7), chlorogenic acid methyl ester (8), 3-O-caffeoyl-5-O-coumaroylquinic acid or 3-O-coumaroyl-5-O-caffeoylquinic acid (9), gallic acid (10), and protocatechuic acid (11).

The current study pioneers to identify and report all the phenolic constituents from P.

cerasifera Ldb leaves.