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Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis
Joint Authors
Thomaz Soccol, Vanete
Soccol, Carlos Ricardo
Link, Juliana Seger
Alban, Silvana Maria
Pereira, Gilberto Vinicius Melo
Source
Journal of Immunology Research
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
This work’s goal was to research new candidate antigens for cutaneous leishmaniosis (CL).
In order to reach the goal, we used random peptide phage display libraries screened using antibodies from Leishmania braziliensis patients.
After selection, three peptides (P1, P2, and P3) were synthesized using Fmoc chemistry.
The peptides individually or a mixture of them (MIX) was subsequently emulsified in complete and incomplete Freund’s adjuvant and injected subcutaneously in golden hamsters.
Sera from the hamsters administered with P1 presented antibodies that recognized proteins between 76 and 150 kDa from L.
braziliensis.
Sera from hamsters which had peptides P2 and P3, as well as the MIX, administered presented antibodies that recognized proteins between 52 and 76 kDa of L.
braziliensis.
The research on the similarity of the peptides’ sequences in protein databases showed that they match a 63 kDa glycoprotein.
The three peptides and the MIX were recognized by the sera from CL patients by immunoassay approach (ELISA).
The peptides’ MIX showed the best performance (79% sensitivity) followed by the P1 (72% sensitivity), and the AS presented 91% sensitivity.
These results show a new route for discovering molecules for diagnosis or for immunoprotection against leishmaniosis.
American Psychological Association (APA)
Link, Juliana Seger& Alban, Silvana Maria& Soccol, Carlos Ricardo& Pereira, Gilberto Vinicius Melo& Thomaz Soccol, Vanete. 2017. Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis. Journal of Immunology Research،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1181967
Modern Language Association (MLA)
Link, Juliana Seger…[et al.]. Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis. Journal of Immunology Research No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1181967
American Medical Association (AMA)
Link, Juliana Seger& Alban, Silvana Maria& Soccol, Carlos Ricardo& Pereira, Gilberto Vinicius Melo& Thomaz Soccol, Vanete. Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis. Journal of Immunology Research. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1181967
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1181967