![](/images/graphics-bg.png)
Generation of Monoclonal Antibodies against Immunoglobulin Proteins of the Domestic Ferret (Mustela putorius furo)
Joint Authors
Kirchenbaum, G. A.
Ross, Ted M.
Source
Journal of Immunology Research
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-14
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The domestic ferret (Mustela putorius furo) serves as an animal model for the study of several viruses that cause human disease, most notably influenza.
Despite the importance of this animal model, characterization of the immune response by flow cytometry (FCM) is severely hampered due to the limited number of commercially available reagents.
To begin to address this unmet need and to facilitate more in-depth study of ferret B cells including the identification of antibody-secreting cells, eight unique murine monoclonal antibodies (mAb) with specificity for ferret immunoglobulin (Ig) were generated using conventional B cell hybridoma technology.
These mAb were screened for reactivity against ferret peripheral blood mononuclear cells by FCM and demonstrate specificity for CD79β+ B cells.
Several of these mAb are specific for the light chain of surface B cell receptor (BCR) and enable segregation of kappa and lambda B cells.
Additionally, a mAb that yielded surface staining of nearly all surface BCR positive cells (i.e., pan ferret Ig) was generated.
Collectively, these MαF-Ig mAb offer advancement compared to the existing portfolio of polyclonal anti-ferret Ig detection reagents and should be applicable to a wide array of immunologic assays including the identification of antibody-secreting cells by FCM.
American Psychological Association (APA)
Kirchenbaum, G. A.& Ross, Ted M.. 2017. Generation of Monoclonal Antibodies against Immunoglobulin Proteins of the Domestic Ferret (Mustela putorius furo). Journal of Immunology Research،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1181970
Modern Language Association (MLA)
Kirchenbaum, G. A.& Ross, Ted M.. Generation of Monoclonal Antibodies against Immunoglobulin Proteins of the Domestic Ferret (Mustela putorius furo). Journal of Immunology Research No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1181970
American Medical Association (AMA)
Kirchenbaum, G. A.& Ross, Ted M.. Generation of Monoclonal Antibodies against Immunoglobulin Proteins of the Domestic Ferret (Mustela putorius furo). Journal of Immunology Research. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1181970
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1181970