Protective Effects of Moringa oleifera on HBV Genotypes C and H Transiently Transfected Huh7 Cells
Joint Authors
Armendáriz-Borunda, Juan
Sandoval-Rodríguez, Ana
Feustel, Sina
Ayón-Pérez, Fabiola
Rodríguez-Echevarría, Roberto
Contreras-Salinas, Homero
Sánchez-Orozco, L. V.
Source
Journal of Immunology Research
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Chronic hepatitis B infection treatment implicates a long-lasting treatment.
M.
oleifera extracts contain compounds with antiviral, antioxidant, and antifibrotic properties.
In this study, the effect of M.
oleifera was evaluated in Huh7 cells expressing either HBV genotypes C or H for the antiviral, antifibrotic, anti-inflammatory, and antioxidative responses.
Huh7 cells were treated with an aqueous extract of M.
oleifera (leaves) at doses of 0, 30, 45, or 60 μg/mL.
The replicative virus and TGF-β1, CTGF, CAT, IFN-β1, and pgRNA expressions were measured by real time.
HBsAg and IL-6 titers were determined by ELISA.
CTGF, TGF-β1, IFN-β1, and pgRNA expressions decreased with M.
oleifera treatment irrespective of the HBV genotype.
HBsAg secretion in the supernatant of transfected Huh7 cells with both HBV genotypes was decreased regardless of the dose of M.
oleifera.
Similar effect was observed in proinflammatory cytokine IL-6, which had a tendency to decrease at 24 hours of treatment.
Transfection with both HBV genotypes strongly decreased CAT expression, which is retrieved with M.
oleifera treatment.
M.
oleifera treatment reduced fibrosis markers, IL-6, and HBsAg secretion in HBV genotypes C and H.
However, at the level of replication, only HBV-DNA genotype C was slightly reduced with this treatment.
American Psychological Association (APA)
Feustel, Sina& Ayón-Pérez, Fabiola& Sandoval-Rodríguez, Ana& Rodríguez-Echevarría, Roberto& Contreras-Salinas, Homero& Armendáriz-Borunda, Juan…[et al.]. 2017. Protective Effects of Moringa oleifera on HBV Genotypes C and H Transiently Transfected Huh7 Cells. Journal of Immunology Research،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1181980
Modern Language Association (MLA)
Feustel, Sina…[et al.]. Protective Effects of Moringa oleifera on HBV Genotypes C and H Transiently Transfected Huh7 Cells. Journal of Immunology Research No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1181980
American Medical Association (AMA)
Feustel, Sina& Ayón-Pérez, Fabiola& Sandoval-Rodríguez, Ana& Rodríguez-Echevarría, Roberto& Contreras-Salinas, Homero& Armendáriz-Borunda, Juan…[et al.]. Protective Effects of Moringa oleifera on HBV Genotypes C and H Transiently Transfected Huh7 Cells. Journal of Immunology Research. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1181980
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1181980