Stability-Indicating High-Performance Liquid Chromatographic Determination of Levamisole Hydrochloride in Bulk and Dosage Forms
Joint Authors
Abdalraheem, Amal
Shantier, Shaza
Abureid, Imad
Gadkariem, Elrasheed A.
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A selective, accurate, and precise stability-indicating HPLC method for analysis of levamisole hydrochloride in bulk and in their injection and oral solution formulations has been developed and validated in accordance with ICH guidelines.
The chromatographic separation was carried out on a C8 column (250 × 4.6 mm with a particle size of 5 micrometer) using a mixture of phosphate buffer pH 8.0 and acetonitrile (70 : 30 v/v) as the mobile phase pumped at a flow rate of 1.5 ml/min with UV detection at 215 nm.
The calibration curve was linear over the 10–50 μg/ml concentration range with a correlation coefficient of 1.0000.
The limit of detection (LOD) and the limit of quantitation (LOQ) were 0.29 μg/ml and 0.89 μg/ml, respectively.
The accuracy and the precision of the developed method were significantly good (RSD < 2%).
The validity of the proposed method was further confirmed through the statistical comparison of the obtained data with those of the official method.
American Psychological Association (APA)
Abdalraheem, Amal& Shantier, Shaza& Abureid, Imad& Gadkariem, Elrasheed A.. 2020. Stability-Indicating High-Performance Liquid Chromatographic Determination of Levamisole Hydrochloride in Bulk and Dosage Forms. Journal of Chemistry،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1181985
Modern Language Association (MLA)
Abdalraheem, Amal…[et al.]. Stability-Indicating High-Performance Liquid Chromatographic Determination of Levamisole Hydrochloride in Bulk and Dosage Forms. Journal of Chemistry No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1181985
American Medical Association (AMA)
Abdalraheem, Amal& Shantier, Shaza& Abureid, Imad& Gadkariem, Elrasheed A.. Stability-Indicating High-Performance Liquid Chromatographic Determination of Levamisole Hydrochloride in Bulk and Dosage Forms. Journal of Chemistry. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1181985
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1181985