Cxcl8b and Cxcr2 Regulate Neutrophil Migration through Bloodstream in Zebrafish
Joint Authors
Reyes, Ariel E.
Feijóo, Carmen G.
Zuñiga-Traslaviña, Constanza
Bravo, Karina
Source
Journal of Immunology Research
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-31
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Neutrophils play an essential role during an inflammatory response, which is dependent on their rapid recruitment from the bone marrow to the vasculature.
However, there is no information about the molecular signals that regulate neutrophil entry to circulation during an inflammatory process in humans.
This is mainly due to the lack of a suitable model of study that contains similar set of molecules and that allows in vivo analyses.
In this study, we used the zebrafish to assess the role of Cxcl8a, Cxcl8b, and Cxcr2 in neutrophil migration to blood circulation after injury.
Using Tg(BACmpx:GFP)i114 transgenic embryos and two damage models (severe and mild), we developed in vivo lack of function assays.
We found that the transcription levels of cxcl8a, cxcl8b, and cxcr2 were upregulated in the severe damage model.
In contrast, only cxcr2 and cxcl8a mRNA levels were increased during mild damage.
After knocking down Cxcl8a, neutrophil quantity decreased at the injury site, while Cxcl8b decreased neutrophils in circulation.
When inhibiting Cxcr2, we observed a decrease in neutrophil entry to the bloodstream.
In conclusion, we identified different functions for both Cxcl8 paralogues, being the Cxcl8b/Cxcr2 axis that regulates neutrophil entry to the bloodstream, while Cxcl8a/Cxcr2 regulates the migration to the affected area.
American Psychological Association (APA)
Zuñiga-Traslaviña, Constanza& Bravo, Karina& Reyes, Ariel E.& Feijóo, Carmen G.. 2017. Cxcl8b and Cxcr2 Regulate Neutrophil Migration through Bloodstream in Zebrafish. Journal of Immunology Research،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1182013
Modern Language Association (MLA)
Zuñiga-Traslaviña, Constanza…[et al.]. Cxcl8b and Cxcr2 Regulate Neutrophil Migration through Bloodstream in Zebrafish. Journal of Immunology Research No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1182013
American Medical Association (AMA)
Zuñiga-Traslaviña, Constanza& Bravo, Karina& Reyes, Ariel E.& Feijóo, Carmen G.. Cxcl8b and Cxcr2 Regulate Neutrophil Migration through Bloodstream in Zebrafish. Journal of Immunology Research. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1182013
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1182013