Simple Label-Free Electrochemical Immunosensor in a Microchamber for Detecting Newcastle Disease Virus
Joint Authors
Tran, Thi Luyen
Chu, Thi Xuan
Tran, Thinh Quang
Ho, Ha Phu
Mai, Tuan Anh
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-10-10
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
In this study, a simple, label-free, electrochemical immunosensor system, including a three-electrode transducer and a microchamber, was designed, fabricated, and integrated with focus toward the detection of Newcastle disease virus (NDV).
The chicken egg yolk antibodies (IgY) against NDV were used as the biological recognition element, replacing purified IgG antibodies that require a complex extraction process and time-consuming.
The IgY against NDV was immobilized on the sensor surface using PrA/GA and SAM/NHS approaches.
The immunosensor showed high sensitivity with NDV concentrations ranging from 106 to 102 EID50/mL with good specificity, repeatability, and small standard deviations.
Compared to traditional methods, the immunosensor with advantages such as simple fabrication, quick response, direct detection, and possibility for miniaturization by integrating the immunosensor with the microchamber is potential for applications in contamination studies and field measurements.
American Psychological Association (APA)
Tran, Thi Luyen& Tran, Thinh Quang& Ho, Ha Phu& Chu, Thi Xuan& Mai, Tuan Anh. 2019. Simple Label-Free Electrochemical Immunosensor in a Microchamber for Detecting Newcastle Disease Virus. Journal of Nanomaterials،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1182110
Modern Language Association (MLA)
Tran, Thi Luyen…[et al.]. Simple Label-Free Electrochemical Immunosensor in a Microchamber for Detecting Newcastle Disease Virus. Journal of Nanomaterials No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1182110
American Medical Association (AMA)
Tran, Thi Luyen& Tran, Thinh Quang& Ho, Ha Phu& Chu, Thi Xuan& Mai, Tuan Anh. Simple Label-Free Electrochemical Immunosensor in a Microchamber for Detecting Newcastle Disease Virus. Journal of Nanomaterials. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1182110
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1182110