Kinetics Investigation on Mushroom Tyrosinase Inhibition of Proso Millet

Abstract EN

Proso millet (Panicum miliaceum) is rich in nutritive components and is widely used as a human food, feed and forage for animals, and fuel.

This study investigated the effect of a proso millet extract on the inhibition of tyrosinase, a key enzyme in melanogenesis.

High performance liquid chromatography analysis indicated that the proso millet extract contained phenolic tyrosinase inhibitors, such as syringic acid, p-coumaric acid, and ferulic acid.

The extract had an IC50 for inhibition of tyrosinase activity of 14.02 mg/mL.

A Lineweaver-Burk double reciprocal plot showed that the proso millet extract functioned as a mixed competitive and noncompetitive inhibitor.

Proso millet has potential as a tyrosinase inhibitor that may have applications in the cosmetics industry.