Transglycosylation Properties of a Novel α-1,4-Glucanotransferase from Bacteroides thetaiotaomicron and Its Application in Developing an α-Glucosidase-Specific Inhibitor
Joint Authors
Choi, Hye-Jeong
Ko, Dam-Seul
Kim, Na-Ri
Choung, Woo-Jae
Koo, Ye-Seul
Jeong, Da-Woon
Shim, Jae-Hoon
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-17
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
In this study, α-glucanotransferase from Bacteroides thetaiotaomicron was expressed in Escherichia coli and characterized.
Conserved amino-acid sequence alignment showed that Bacteroides thetaiotaomicron α-glucanotransferase (BtαGTase) belongs to the glycoside hydrolase family 77.
The enzyme exhibited optimal catalytic activity at 60°C and pH 3.0.
BtαGTase catalyzed transglycosylation reactions that produced only glycosyl or maltosyl transfer products, which are preferable for the generation of transglycosylated products with high yield.
The 1-deoxynojirimycin (DNJ) glycosylation product G1-DNJ was generated using BtαGTase, and the inhibitory effect of G1-DNJ was analyzed.
A kinetic study of inhibition revealed that G1-DNJ inhibited α-glucosidase to a greater extent than did DNJ but did not show any inhibitory effects towards α-amylase, suggesting that G1-DNJ is a potential candidate for the prevention of diabetes.
American Psychological Association (APA)
Choi, Hye-Jeong& Ko, Dam-Seul& Kim, Na-Ri& Choung, Woo-Jae& Koo, Ye-Seul& Jeong, Da-Woon…[et al.]. 2018. Transglycosylation Properties of a Novel α-1,4-Glucanotransferase from Bacteroides thetaiotaomicron and Its Application in Developing an α-Glucosidase-Specific Inhibitor. Journal of Chemistry،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1182229
Modern Language Association (MLA)
Choi, Hye-Jeong…[et al.]. Transglycosylation Properties of a Novel α-1,4-Glucanotransferase from Bacteroides thetaiotaomicron and Its Application in Developing an α-Glucosidase-Specific Inhibitor. Journal of Chemistry No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1182229
American Medical Association (AMA)
Choi, Hye-Jeong& Ko, Dam-Seul& Kim, Na-Ri& Choung, Woo-Jae& Koo, Ye-Seul& Jeong, Da-Woon…[et al.]. Transglycosylation Properties of a Novel α-1,4-Glucanotransferase from Bacteroides thetaiotaomicron and Its Application in Developing an α-Glucosidase-Specific Inhibitor. Journal of Chemistry. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1182229
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1182229