Inflammatory Cytokine Pattern Is Sex-Dependent in Mouse Cutaneous Melanoma Experimental Model
Joint Authors
Zurac, Sabina
Căruntu, Constantin
Surcel, Mihaela
Constantin, Carolina
Neagu, Monica
Source
Journal of Immunology Research
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
We present the evaluation of inflammatory cytokines in mouse cutaneous melanoma experimental model, as markers of disease evolution.
Moreover, to test our experimental model, we have used low doses of dacarbazine (DTIC).
C57 BL/6J mouse of both sexes were subjected to experimental cutaneous melanoma and treated with low doses of DTIC.
Clinical parameters and serum cytokines were followed during tumor evolution and during DTIC therapy.
Cytokine/chemokine pattern was assessed using xMAP technology and the following molecules were quantified: interleukins (IL)-1-beta, IL-6, IL-10, IL-12 (p70), interferon (IFN)-gamma, granulocyte macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor (TNF)-alpha, macrophage inflammatory protein (MIP)-1alpha, monocyte chemoattractant protein (MCP-1), and keratinocyte-derived chemokine (KC).
Significant differences were found between normal females and males mice, female mice having a statistically higher serum concentration of IL-1-beta compared to male mice, while males have a significantly higher concentration of MIP-1-alpha.
During melanoma evolution in the female group, IL-1-beta, MIP-1-alpha, and KC circulatory levels were found 10-fold increased, while other cytokines doubled their values.
In the male mice group, only circulatory KC increased 4 times, while IL-1-beta and TNF-alpha doubled their circulatory values.
Various serum cytokines correlated with the disease evolution in cutaneous melanoma mouse model.
American Psychological Association (APA)
Surcel, Mihaela& Constantin, Carolina& Căruntu, Constantin& Zurac, Sabina& Neagu, Monica. 2017. Inflammatory Cytokine Pattern Is Sex-Dependent in Mouse Cutaneous Melanoma Experimental Model. Journal of Immunology Research،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1182237
Modern Language Association (MLA)
Surcel, Mihaela…[et al.]. Inflammatory Cytokine Pattern Is Sex-Dependent in Mouse Cutaneous Melanoma Experimental Model. Journal of Immunology Research No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1182237
American Medical Association (AMA)
Surcel, Mihaela& Constantin, Carolina& Căruntu, Constantin& Zurac, Sabina& Neagu, Monica. Inflammatory Cytokine Pattern Is Sex-Dependent in Mouse Cutaneous Melanoma Experimental Model. Journal of Immunology Research. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1182237
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1182237