Gamma-Tocotrienol Stimulates the Proliferation, Differentiation, and Mineralization in Osteoblastic MC3T3-E1 Cells
Joint Authors
Zhou, Shaobo
Xu, Weili
He, Pan
He, Shenghua
Cui, Pengju
Mi, Yaqing
Yang, Yang
Li, Yang
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-15
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Gamma-tocotrienol, a major component of tocotrienol-rich fraction of palm oil, has been suggested to exhibit bone protective effects in vivo.
However, the effects of γ-tocotrienol on osteoblast cells are still unclear.
In this study, the effects of γ-tocotrienol on the proliferation, differentiation, and mineralization in osteoblastic MC3T3-E1 cells were investigated.
Our results showed that γ-tocotrienol (2–8 μmol/L) significantly improved the cell proliferation (p<0.05), but it did not affect cell cycle progression.
γ-Tocotrienol significantly increased alkaline phosphatase (ALP) activity (p<0.05), secretion levels of osteocalcin (OC) and osteonectin (ON), and mRNA levels of collagen type I (Col I) of MC3T3-E1 cells.
Meanwhile, we found that γ-tocotrienol is promoted in differentiation MC3T3-E1 cells by upregulation of the expression of Runx2 protein.
Moreover, the number of bone nodules increased over 2.5-fold in cells treated with γ-tocotrienol (2–8 μmol/L) for 24 d compared to control group.
These results indicated that γ-tocotrienol at low dose levels, especially 4 μmol/L, could markedly enhance the osteoblastic function by increasing the proliferation, differentiation, and mineralization of osteoblastic MC3T3-E1 cells.
Moreover, our data also indicated that Runx2 protein may be involved in these effects.
Further studies are needed to determine the potential of γ-tocotrienol as an antiosteoporotic agent.
American Psychological Association (APA)
Xu, Weili& He, Pan& He, Shenghua& Cui, Pengju& Mi, Yaqing& Yang, Yang…[et al.]. 2018. Gamma-Tocotrienol Stimulates the Proliferation, Differentiation, and Mineralization in Osteoblastic MC3T3-E1 Cells. Journal of Chemistry،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1182291
Modern Language Association (MLA)
Xu, Weili…[et al.]. Gamma-Tocotrienol Stimulates the Proliferation, Differentiation, and Mineralization in Osteoblastic MC3T3-E1 Cells. Journal of Chemistry No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1182291
American Medical Association (AMA)
Xu, Weili& He, Pan& He, Shenghua& Cui, Pengju& Mi, Yaqing& Yang, Yang…[et al.]. Gamma-Tocotrienol Stimulates the Proliferation, Differentiation, and Mineralization in Osteoblastic MC3T3-E1 Cells. Journal of Chemistry. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1182291
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1182291