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Relevance and Standardization of In Vitro Antioxidant Assays: ABTS, DPPH, and Folin–Ciocalteu
Joint Authors
Cigić, Blaž
Abramovič, Helena
Grobin, Blaž
Ulrih, Nataša Poklar
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Trolox, gallic acid, chlorogenic acid, caffeic acid, catechin, epigallocatechin gallate, and ascorbic acid are antioxidants used as standards for reaction with chromogenic radicals, 2,2-diphenyl-1-picrylhydrazyl (DPPH⋅) and 2,2′-azino-bis-3-ethylbenzotiazolin-6-sulfonic acid (ABTS⋅+), and Folin–Ciocalteu (FC) reagent.
The number of exchanged electrons has been analyzed as function of method and solvent.
A majority of compounds exchange more electrons in FC assay than in ABTS and DPPH assays.
In reaction with chromogenic radicals, the largest number of electrons was exchanged in buffer (pH 7.4) and the lowest reactivity was in methanol (DPPH) and water (ABTS).
At physiological pH, the number of exchanged electrons of polyphenols exceeded the number of OH groups, pointing to the important contribution of partially oxidized antioxidants, formed in the course of reaction, to the antioxidant potential.
For Trolox, small impact on the number of exchanged electrons was observed, confirming that it is more suitable as a standard compound than the other antioxidants.
American Psychological Association (APA)
Abramovič, Helena& Grobin, Blaž& Ulrih, Nataša Poklar& Cigić, Blaž. 2018. Relevance and Standardization of In Vitro Antioxidant Assays: ABTS, DPPH, and Folin–Ciocalteu. Journal of Chemistry،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1182360
Modern Language Association (MLA)
Abramovič, Helena…[et al.]. Relevance and Standardization of In Vitro Antioxidant Assays: ABTS, DPPH, and Folin–Ciocalteu. Journal of Chemistry No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1182360
American Medical Association (AMA)
Abramovič, Helena& Grobin, Blaž& Ulrih, Nataša Poklar& Cigić, Blaž. Relevance and Standardization of In Vitro Antioxidant Assays: ABTS, DPPH, and Folin–Ciocalteu. Journal of Chemistry. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1182360
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1182360