Preparation of Carbon Dots with High-Fluorescence Quantum Yield and Their Application in Dopamine Fluorescence Probe and Cellular Imaging
Joint Authors
Lin, Haitao
Huang, Jun
Ding, Liyun
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-10-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Carbon dots represent a kind of fluorescent nanomaterial and have broad application prospects in the field of biosensing and optoelectronics.
Here, we explored carbon dots with a high-fluorescence quantum yield rate synthesized from L-cysteine and citric acid by the microwave-assisted method.
The characteristics of the carbon dots were studied using a transmission electron microscope, an X-ray diffractometer, X-ray photoelectron spectra, a UV-Vis absorption spectrometer, a FTIR spectrometer, and a fluorescence spectrophotometer.
The obtained carbon dots exhibited a high-fluorescence quantum yield (up to 85%), which is due to the combination of amidogens and sulfydryl with carbon dots, and hence bring the improved fluorescence property.
We used carbon dots for in vitro imaging of CRL-5822 cells and human umbilical vein endothelial cells, which showed the low inhibitory rate (0.8%) of cells for 48 h with good biocompatibility demonstrated by the cell viability assay.
The image of cells can be observed clearly under UV light.
The Stern-Volmer equation was introduced to describe the quenching effect between the fluorescence intensity of carbon dots and the concentration of aqueous dopamine (DA).
American Psychological Association (APA)
Lin, Haitao& Huang, Jun& Ding, Liyun. 2019. Preparation of Carbon Dots with High-Fluorescence Quantum Yield and Their Application in Dopamine Fluorescence Probe and Cellular Imaging. Journal of Nanomaterials،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1182414
Modern Language Association (MLA)
Lin, Haitao…[et al.]. Preparation of Carbon Dots with High-Fluorescence Quantum Yield and Their Application in Dopamine Fluorescence Probe and Cellular Imaging. Journal of Nanomaterials No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1182414
American Medical Association (AMA)
Lin, Haitao& Huang, Jun& Ding, Liyun. Preparation of Carbon Dots with High-Fluorescence Quantum Yield and Their Application in Dopamine Fluorescence Probe and Cellular Imaging. Journal of Nanomaterials. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1182414
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1182414