Cell-SELEX Identifies a “Sticky” RNA Aptamer Sequence
Joint Authors
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Cell-SELEX is performed to select for cell binding aptamers.
We employed an additional selection pressure by using RNAse to remove surface-binding aptamers and select for cell-internalizing aptamers.
A common RNA sequence was identified from independent cell-SELEX procedures against two different pancreatic cancer cell lines, indicating a strong selection pressure towards this sequence from the large pool of other available sequences present in the aptamer library.
The aptamer is not specific for the pancreatic cancer cell lines, and a similar sequence motif is present in previously published internalizing aptamers.
The identified sequence forms a structural motif that binds to a surface protein, which either is highly abundant or has strong affinity for the selected aptamer sequence.
Deselecting (removing) this sequence during cell-SELEX may increase the probability of identifying aptamers against cell type-specific targets on the cell surface.
American Psychological Association (APA)
Ray, Partha& White, Rebekah R.. 2017. Cell-SELEX Identifies a “Sticky” RNA Aptamer Sequence. Journal of Nucleic Acids،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1182455
Modern Language Association (MLA)
Ray, Partha& White, Rebekah R.. Cell-SELEX Identifies a “Sticky” RNA Aptamer Sequence. Journal of Nucleic Acids No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1182455
American Medical Association (AMA)
Ray, Partha& White, Rebekah R.. Cell-SELEX Identifies a “Sticky” RNA Aptamer Sequence. Journal of Nucleic Acids. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1182455
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1182455