The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding
Joint Authors
Leeder, W.-Matthias
Voskuhl, Stephan
Göringer, H. Ulrich
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Mitochondrial transcript maturation in African trypanosomes requires RNA editing to convert sequence-deficient pre-mRNAs into translatable mRNAs.
The different pre-mRNAs have been shown to adopt highly stable 2D folds; however, it is not known whether these structures resemble the in vivo folds given the extreme “crowding” conditions within the mitochondrion.
Here, we analyze the effects of macromolecular crowding on the structure of the mitochondrial RPS12 pre-mRNA.
We use high molecular mass polyethylene glycol as a macromolecular cosolute and monitor the structure of the RNA globally and with nucleotide resolution.
We demonstrate that crowding has no impact on the 2D fold and we conclude that the MFE structure in dilute solvent conditions represents a good proxy for the folding of the pre-mRNA in its mitochondrial solvent context.
American Psychological Association (APA)
Leeder, W.-Matthias& Voskuhl, Stephan& Göringer, H. Ulrich. 2017. The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding. Journal of Nucleic Acids،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1182457
Modern Language Association (MLA)
Leeder, W.-Matthias…[et al.]. The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding. Journal of Nucleic Acids No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1182457
American Medical Association (AMA)
Leeder, W.-Matthias& Voskuhl, Stephan& Göringer, H. Ulrich. The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding. Journal of Nucleic Acids. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1182457
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1182457