The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding

Joint Authors

Leeder, W.-Matthias
Voskuhl, Stephan
Göringer, H. Ulrich

Source

Journal of Nucleic Acids

Issue

Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2017-06-18

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Biology
Medicine

Abstract EN

Mitochondrial transcript maturation in African trypanosomes requires RNA editing to convert sequence-deficient pre-mRNAs into translatable mRNAs.

The different pre-mRNAs have been shown to adopt highly stable 2D folds; however, it is not known whether these structures resemble the in vivo folds given the extreme “crowding” conditions within the mitochondrion.

Here, we analyze the effects of macromolecular crowding on the structure of the mitochondrial RPS12 pre-mRNA.

We use high molecular mass polyethylene glycol as a macromolecular cosolute and monitor the structure of the RNA globally and with nucleotide resolution.

We demonstrate that crowding has no impact on the 2D fold and we conclude that the MFE structure in dilute solvent conditions represents a good proxy for the folding of the pre-mRNA in its mitochondrial solvent context.

American Psychological Association (APA)

Leeder, W.-Matthias& Voskuhl, Stephan& Göringer, H. Ulrich. 2017. The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding. Journal of Nucleic Acids،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1182457

Modern Language Association (MLA)

Leeder, W.-Matthias…[et al.]. The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding. Journal of Nucleic Acids No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1182457

American Medical Association (AMA)

Leeder, W.-Matthias& Voskuhl, Stephan& Göringer, H. Ulrich. The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding. Journal of Nucleic Acids. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1182457

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1182457