Putative HIV and SIV G-Quadruplex Sequences in Coding and Noncoding Regions Can Form G-Quadruplexes
Joint Authors
Viglasky, Viktor
Krafčíková, Petra
Demkovičová, Erika
Halaganová, Andrea
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-12-31
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The HIV virus is one of the most studied viruses in the world.
This is especially true in terms of gene sequencing, and to date more than 9 thousand genomic sequences of HIV isolates have been sequenced and analyzed.
In this study, a series of DNA sequences, which have the potential to form G-quadruplex structures, is analyzed.
Several such sequences were found in various coding and noncoding virus domains, including the U3 LTR, tat, rev, env, and vpx regions.
Interestingly, a homological sequence to the already well-known HIV integrase aptamer was identified in the minus-strand.
The sequences derived from original isolates were analyzed using standard spectral and electrophoretic methods.
In addition, a recently developed methodology is applied which uses induced circular dichroism spectral profiles of G-quadruplex-ligand (Thiazole Orange) complexes to determine if G-rich sequences can adopt G-quadruplex structure.
Targeting the G-quadruplexes or peptide domains corresponding to the G-rich coding sequence in HIV offers researchers attractive therapeutic targets which would be of particular use in the development of novel antiviral therapies.
The analysis of G-rich regions can provide researchers with a path to find specific targets which could be of interest for specific types of virus.
American Psychological Association (APA)
Krafčíková, Petra& Demkovičová, Erika& Halaganová, Andrea& Viglasky, Viktor. 2017. Putative HIV and SIV G-Quadruplex Sequences in Coding and Noncoding Regions Can Form G-Quadruplexes. Journal of Nucleic Acids،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1182464
Modern Language Association (MLA)
Krafčíková, Petra…[et al.]. Putative HIV and SIV G-Quadruplex Sequences in Coding and Noncoding Regions Can Form G-Quadruplexes. Journal of Nucleic Acids No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1182464
American Medical Association (AMA)
Krafčíková, Petra& Demkovičová, Erika& Halaganová, Andrea& Viglasky, Viktor. Putative HIV and SIV G-Quadruplex Sequences in Coding and Noncoding Regions Can Form G-Quadruplexes. Journal of Nucleic Acids. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1182464
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1182464