Selection of DNA Aptamers for Ovarian Cancer Biomarker CA125 Using One-Pot SELEX and High-Throughput Sequencing
Joint Authors
Scoville, Delia J.
Uhm, Tae Kyu Brian
Shallcross, Jamie A.
Whelan, Rebecca J.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
CA125 is a mucin glycoprotein whose concentration in serum correlates with a woman’s risk of developing ovarian cancer and also indicates response to therapy in diagnosed patients.
Accurate detection of this large, complex protein in patient samples is of great clinical relevance.
We suggest that powerful new diagnostic tools may be enabled by the development of nucleic acid aptamers with affinity for CA125.
Here, we report on our use of One-Pot SELEX to isolate single-stranded DNA aptamers with affinity for CA125, followed by high-throughput sequencing of the selected oligonucleotides.
This data-rich approach, combined with bioinformatics tools, enabled the entire selection process to be characterized.
Using fluorescence anisotropy and affinity probe capillary electrophoresis, the binding affinities of four aptamer candidates were evaluated.
Two aptamers, CA125_1 and CA125_12, both without primers, were found to bind to clinically relevant concentrations of the protein target.
Binding was differently influenced by the presence of Mg2+ ions, being required for binding of CA125_1 and abrogating binding of CA125_12.
In conclusion, One-Pot SELEX was found to be a promising selection method that yielded DNA aptamers to a clinically important protein target.
American Psychological Association (APA)
Scoville, Delia J.& Uhm, Tae Kyu Brian& Shallcross, Jamie A.& Whelan, Rebecca J.. 2017. Selection of DNA Aptamers for Ovarian Cancer Biomarker CA125 Using One-Pot SELEX and High-Throughput Sequencing. Journal of Nucleic Acids،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1182493
Modern Language Association (MLA)
Scoville, Delia J.…[et al.]. Selection of DNA Aptamers for Ovarian Cancer Biomarker CA125 Using One-Pot SELEX and High-Throughput Sequencing. Journal of Nucleic Acids No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1182493
American Medical Association (AMA)
Scoville, Delia J.& Uhm, Tae Kyu Brian& Shallcross, Jamie A.& Whelan, Rebecca J.. Selection of DNA Aptamers for Ovarian Cancer Biomarker CA125 Using One-Pot SELEX and High-Throughput Sequencing. Journal of Nucleic Acids. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1182493
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1182493