Preliminary Characterization of a Homogeneous Polysaccharide with Anticomplement Activity from Sijunzi Decoction
Joint Authors
Li, Xiaobo
Wang, Ruijun
Peng, Ying
Ji, Yunfei
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Sijunzi decoction (SJZD) is a classical herbal prescription in traditional Chinese medicine (TCM) used for enhancing the function of immune system.
In previous studies, a polysaccharide fraction S-3 was screened from SJZD by assessment of immune system regulation, intestinal microbiota, and SCFA in order to explore the immune active ingredients in SJZD.
In the present study, S-3 was further purified, and a homogeneous polysaccharide S-3-1 with a molecular mass of 13.5 × 104 Da was obtained after further fractionation by Sephadex G-150 size-exclusion chromatography.
The immunological activities of S-3-1 were assayed in vitro for the first time.
The determination of the anticomplement activity showed that S-3-1 displayed inhibitory effects on classical pathway of the complement system, with CH50 values of 530 μg/mL.
The FT-IR analysis showed that S-3-1 had absorptive peaks characteristic of polysaccharides.
The methylation and GC-MS analysis showed that it is comprised of Rha, Ara, Xyl, Man, Gal, and Glc in a relative molar ratio of 0.35 : 0.37 : 1.4 : 0.31 : 3 : 0.8 and that it mainly contained 1,4-linked-Glc and 1,6-linked-Gal glycosidic bonds.
The morphology of S-3-1 was observed by atomic force microscope (AFM).
These results provided evidences for tracking the material basis of SJZD immune activity.
American Psychological Association (APA)
Wang, Ruijun& Ji, Yunfei& Peng, Ying& Li, Xiaobo. 2018. Preliminary Characterization of a Homogeneous Polysaccharide with Anticomplement Activity from Sijunzi Decoction. Journal of Chemistry،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1182662
Modern Language Association (MLA)
Wang, Ruijun…[et al.]. Preliminary Characterization of a Homogeneous Polysaccharide with Anticomplement Activity from Sijunzi Decoction. Journal of Chemistry No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1182662
American Medical Association (AMA)
Wang, Ruijun& Ji, Yunfei& Peng, Ying& Li, Xiaobo. Preliminary Characterization of a Homogeneous Polysaccharide with Anticomplement Activity from Sijunzi Decoction. Journal of Chemistry. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1182662
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1182662