A Chemical Synthesis of Benzo[a]pyrene Hapten and Its Application in a Streptavidin-Horseradish Peroxidase-Based Enzyme-Linked Immunological Analysis

Abstract EN

A specific BaP hapten was synthesized and BaP antigens (immunogen, coating antigen) were prepared using diverse methods.

Based on these works, a streptavidin-horseradish peroxidase-based enzyme-linked immunological analysis was developed and firstly used to detect BaP.

Several physiochemical factors that may influence the assay performance were optimized in the assay.

Under optimal conditions, a detection limit of 0.0094 ng mL−1 was obtained and good linearity was achieved within a range of 0.03–35.40 ng mL−1.

Satisfactory recovery of spiked samples (91.12–109.23%) was obtained and the coefficient of variation was acceptable.

Finally, the detection results of BaP in environmental and food samples were consistent with those obtained using high-performance liquid chromatography.

The proposed immunoassay is reliable and has great potential for detecting trace amounts of BaP.