Insulin-Attenuated Inflammatory Response of Dendritic Cells in Diabetes by Regulating RAGE-PKCβ1-IRS1-NF-κB Signal Pathway: A Study on the Anti-Inflammatory Mechanism of Insulin in Diabetes
Joint Authors
Zou, Yunzeng
Zhao, Liding
Li, Ya
Lv, Qingbo
Wang, Min
Luan, Yi
Song, Jiale
Fu, Guosheng
Ge, Junbo
Zhang, Wenbin
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-30
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Background.
Diabetes is associated with chronic inflammation, and dendritic cells (DCs) have proinflammatory effect in diabetes.
The anti-inflammatory effect of insulin on diabetes is not entirely clear.
The study aims to examine insulin-induced effects on the inflammatory response in DCs.
Methods.
Twenty-one C57BL/6 mice were divided into 3 groups.
Streptozotocin was injected into the diabetic mice model.
The bone marrow-derived DCs (BMDCs) were obtained from C57BL/6 mice.
CD83, CD86, and type II major histocompatibility complex (MHC-II) of BMDCs were measured by flow cytometry.
The fluctuations in the RNA levels of cytokines and chemokines were analyzed by quantitative RT-PCR.
The concentrations of IFN-γ and TNF-α were calculated using ELISA kits, and the proteins were detected using western blot.
Results.
In CD11c+ DCs derived from the spleens with hyperglycemia, the expression of CD83 and CD86 in diabetic mice was significantly upregulated, coupled with a higher secretion level of cytokines and chemokines, and increased phosphorylation of NF-κB and IκB.
Insulin therapy was found to have a reversal effect on the inflammatory response and immune maturation in DCs.
In AGEs-BSA-stimulated BMDCs, insulin repressed the immune maturation and downregulated the expression of RAGE, phospho-PKCβ1, and serine phospho-IRS1 in an adose-dependent manner.
Such effects can be abolished by PMA, but not IR-neutralizing antibody.
AGEs-BSA-induced BMDCs immune maturation was inhibited by the neutralizing antibody of RAGE, the PKCβ1 inhibitor, or the IRS1 siRNA.
Conclusions.
Insulin has the capability of attenuating the inflammatory response of DCs in diabetes, partly through the downregulation of RAGE expression followed by the inhibition of PKCβ1 phosphorylation and IRS1 serine phosphorylation, resulting in the inactivation of IR binding-independent NF-κB.
This might partly explain the antiatherogenic effect of insulin on diabetes.
American Psychological Association (APA)
Zhao, Liding& Li, Ya& Lv, Qingbo& Wang, Min& Luan, Yi& Song, Jiale…[et al.]. 2020. Insulin-Attenuated Inflammatory Response of Dendritic Cells in Diabetes by Regulating RAGE-PKCβ1-IRS1-NF-κB Signal Pathway: A Study on the Anti-Inflammatory Mechanism of Insulin in Diabetes. Journal of Diabetes Research،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1182887
Modern Language Association (MLA)
Zhao, Liding…[et al.]. Insulin-Attenuated Inflammatory Response of Dendritic Cells in Diabetes by Regulating RAGE-PKCβ1-IRS1-NF-κB Signal Pathway: A Study on the Anti-Inflammatory Mechanism of Insulin in Diabetes. Journal of Diabetes Research No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1182887
American Medical Association (AMA)
Zhao, Liding& Li, Ya& Lv, Qingbo& Wang, Min& Luan, Yi& Song, Jiale…[et al.]. Insulin-Attenuated Inflammatory Response of Dendritic Cells in Diabetes by Regulating RAGE-PKCβ1-IRS1-NF-κB Signal Pathway: A Study on the Anti-Inflammatory Mechanism of Insulin in Diabetes. Journal of Diabetes Research. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1182887
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1182887