Amylase-Dependent Regulation of Glucose Metabolism and InsulinGlucagon Secretion in the Streptozotocin-Induced Diabetic Pig Model and in a Rat Pancreatic Beta-Cell Line, BRIN-BD11
Joint Authors
Pierzynowska, Kateryna
Oredsson, Stina
Pierzynowski, Stefan
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-18
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The current study was aimed at highlighting the role of blood pancreatic amylase in the regulation of glucose homeostasis and insulin secretion in a porcine model of streptozotocin- (STZ-) induced diabetes and in a rat pancreatic beta-cell line, BRIN-BD11.
Blood glucose, plasma insulin, and glucagon levels were measured following a duodenal glucose tolerance test (IDGTT), in four pigs with STZ-induced type 2 diabetes (T2D pigs) and in four pigs with STZ-induced type 1 diabetes (T1D pigs).
Four intact pigs were used as the control group.
The effect of amylase supplementation on both acute and chronic insulin secretion was determined in a BRIN-BD11 cell line.
The amylase infusion had no effect on the glucose utilization curve or glucagon levels in the healthy pigs.
However, a significant lowering of insulin release was observed in healthy pigs treated with amylase.
In the T2D pigs, the glucose utilization curve was significantly lowered in the presence of amylase, while the insulin response curve remained unchanged.
Amylase also significantly increased glucagon release during the IDGTT in the T2D and T1D pigs, by between 2- and 4-fold.
Amylase did not affect the glucose utilization curve in the T1D pigs.
Amylase supplementation significantly decreased both acute and chronic insulin secretion in the BRIN-BD11 cells.
These data confirm our previous observations and demonstrate the participation of pancreatic amylase in glucose absorption/utilization.
Moreover, the present study clearly highlights the direct impact of pancreatic blood amylase on insulin secretion from pancreatic beta-cells and its interactions with insulin and glucagon secretion in a porcine model.
American Psychological Association (APA)
Pierzynowska, Kateryna& Oredsson, Stina& Pierzynowski, Stefan. 2020. Amylase-Dependent Regulation of Glucose Metabolism and InsulinGlucagon Secretion in the Streptozotocin-Induced Diabetic Pig Model and in a Rat Pancreatic Beta-Cell Line, BRIN-BD11. Journal of Diabetes Research،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1182935
Modern Language Association (MLA)
Pierzynowska, Kateryna…[et al.]. Amylase-Dependent Regulation of Glucose Metabolism and InsulinGlucagon Secretion in the Streptozotocin-Induced Diabetic Pig Model and in a Rat Pancreatic Beta-Cell Line, BRIN-BD11. Journal of Diabetes Research No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1182935
American Medical Association (AMA)
Pierzynowska, Kateryna& Oredsson, Stina& Pierzynowski, Stefan. Amylase-Dependent Regulation of Glucose Metabolism and InsulinGlucagon Secretion in the Streptozotocin-Induced Diabetic Pig Model and in a Rat Pancreatic Beta-Cell Line, BRIN-BD11. Journal of Diabetes Research. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1182935
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1182935