Polymalic Acid Tritryptophan Copolymer Interacts with Lipid Membrane Resulting in Membrane Solubilization
Joint Authors
Black, Keith L.
Ding, Hui
Fox, Irving
Patil, Rameshwar
Galstyan, Anna
Holler, Eggehard
Ljubimova, Julia Y.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-21
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Anionic polymers with membrane permeation functionalities are highly desirable for secure cytoplasmic drug delivery.
We have developed tritryptophan containing copolymer (P/WWW) of polymalic acid (PMLA) that permeates membranes by a mechanism different from previously described PMLA copolymers of trileucine (P/LLL) and leucine ethyl ester (P/LOEt) that use the “barrel stave” and “carpet” mechanism, respectively.
The novel mechanism leads to solubilization of membranes by forming copolymer “belts” around planar membrane “packages.” The formation of such packages is supported by results obtained from studies including size-exclusion chromatography, confocal microscopy, and fluorescence energy transfer.
According to this “belt” mechanism, it is hypothesized that P/WWW first attaches to the membrane surface.
Subsequently the hydrophobic tryptophan side chains translocate into the periphery and insert into the lipid bilayer thereby cutting the membrane into packages.
The reaction is driven by the high affinity between the tryptophan residues and lipid side chains resulting in a stable configuration.
The formation of the membrane packages requires physical agitation suggesting that the success of the translocation depends on the fluidity of the membrane.
It is emphasized that the “belt” mechanism could specifically function in the recognition of abnormal cells with high membrane fluidity and in response to hyperthermia.
American Psychological Association (APA)
Ding, Hui& Fox, Irving& Patil, Rameshwar& Galstyan, Anna& Black, Keith L.& Ljubimova, Julia Y.…[et al.]. 2017. Polymalic Acid Tritryptophan Copolymer Interacts with Lipid Membrane Resulting in Membrane Solubilization. Journal of Nanomaterials،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1182980
Modern Language Association (MLA)
Ding, Hui…[et al.]. Polymalic Acid Tritryptophan Copolymer Interacts with Lipid Membrane Resulting in Membrane Solubilization. Journal of Nanomaterials No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1182980
American Medical Association (AMA)
Ding, Hui& Fox, Irving& Patil, Rameshwar& Galstyan, Anna& Black, Keith L.& Ljubimova, Julia Y.…[et al.]. Polymalic Acid Tritryptophan Copolymer Interacts with Lipid Membrane Resulting in Membrane Solubilization. Journal of Nanomaterials. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1182980
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1182980