Protective Role of Tangshen Formula on the Progression of Renal Damage in dbdb Mice by TRPC6Talin1 Pathway in Podocytes
Joint Authors
Li, Jialin
Zhang, Haojun
Tian, Xuefei
Zhao, Hailing
Wang, Qian
Zhou, Xuefeng
Li, Ping
Zhou, Wei’e
Zhao, Tingting
Wang, Yan
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-17
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Abstract EN
Tangshen Formula (TSF) is a Chinese Medicine formula that has been reported to alleviate proteinuria and protect renal function in humans and animals with diabetic kidney disease (DKD).
However, little is known about its mechanism in improving proteinuria.
The dysregulation of podocyte cell-matrix adhesion has been demonstrated to play an important role in the pathogenesis and progression of proteinuric kidney diseases including DKD.
In the present study, the underlying protective mechanism of TSF on podocytes was investigated using the murine model of type 2 DKD db/db mice in vivo and advanced glycation end products (AGEs)-stimulated primary mice podocytes in vitro.
Results revealed that TSF treatment could significantly mitigate reduction of podocyte numbers and foot process effacement, reduce proteinuria, and protect renal function in db/db mice.
There was a significant increase in expression of transient receptor potential canonical channel 6 (TRPC6) and a decrease in expression of talin1 in podocytes of db/db mice.
The results of AGEs-stimulated primary mice podocytes showed increased cell migration and actin-cytoskeleton rearrangement.
Moreover, primary mice podocytes stimulated by AGEs displayed an increase in TRPC6-dependent Ca2+ influx, a loss of talin1, and translocation of nuclear factor of activated T cell (NFATC) 2.
These dysregulations in mice primary podocytes stimulated by AGEs could be significantly attenuated after TSF treatment.
1-Oleoyl-2-acetyl-sn-glycerol (OAG), a TRPC6 agonist, blocked the protective role of TSF on podocyte cell-matrix adherence.
In conclusion, TSF could protect podocytes from injury and reduce proteinuria in DKD, which may be mediated by the regulation of the TRPC6/Talin1 pathway in podocytes.
American Psychological Association (APA)
Wang, Qian& Tian, Xuefei& Zhou, Wei’e& Wang, Yan& Zhao, Hailing& Li, Jialin…[et al.]. 2020. Protective Role of Tangshen Formula on the Progression of Renal Damage in dbdb Mice by TRPC6Talin1 Pathway in Podocytes. Journal of Diabetes Research،Vol. 2020, no. 2020, pp.1-19.
https://search.emarefa.net/detail/BIM-1183048
Modern Language Association (MLA)
Wang, Qian…[et al.]. Protective Role of Tangshen Formula on the Progression of Renal Damage in dbdb Mice by TRPC6Talin1 Pathway in Podocytes. Journal of Diabetes Research No. 2020 (2020), pp.1-19.
https://search.emarefa.net/detail/BIM-1183048
American Medical Association (AMA)
Wang, Qian& Tian, Xuefei& Zhou, Wei’e& Wang, Yan& Zhao, Hailing& Li, Jialin…[et al.]. Protective Role of Tangshen Formula on the Progression of Renal Damage in dbdb Mice by TRPC6Talin1 Pathway in Podocytes. Journal of Diabetes Research. 2020. Vol. 2020, no. 2020, pp.1-19.
https://search.emarefa.net/detail/BIM-1183048
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1183048