Mouse Umbilical Cord Mesenchymal Stem Cell Paracrine Alleviates Renal Fibrosis in Diabetic Nephropathy by Reducing Myofibroblast Transdifferentiation and Cell Proliferation and Upregulating MMPs in Mesangial Cells
Joint Authors
Ma, Xiaoqian
Rong, Pengfei
Wang, Wei
Li, Hongde
Nie, Wei
Chen, Yan
Zhang, Juan
Dong, Qiong
Yang, Min
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-05
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Transplantation of umbilical cord mesenchymal stem cells (UC-MSCs) is currently considered a novel therapeutic strategy for diabetic nephropathy (DN).
However, the mechanisms by which UC-MSCs ameliorate renal fibrosis in DN are not well understood.
Herein, we firstly investigated the therapeutic effects of mouse UC-MSC infusion on kidney structural and functional impairment in streptozotocin- (STZ-) induced diabetic mice.
We found that the repeated injection with mUC-MSCs alleviates albuminuria, glomerulus injury, and fibrosis in DN mouse models.
Next, mesangial cells were exposed to 5.6 mM glucose, 30 mM glucose, or mUC-MSC-conditioned medium, and then we performed western blotting, immunofluorescence, wound healing assay, and cell proliferation assay to measure extracellular matrix (ECM) proteins and matrix metalloproteinases (MMPs), myofibroblast transdifferentiation (MFT), and cell proliferation.
We demonstrated that mUC-MSC paracrine decreased the deposition of fibronectin and collagen I by inhibiting TGF-β1-triggered MFT and cell proliferation mediated by PI3K/Akt and MAPK signaling pathways, and elevating the levels of MMP2 and MMP9.
Importantly, we provided evidence that the antifibrosis role of mUC-MSC paracrine in DN might be determined by exosomes shed by MSCs.
Together, these findings reveal the mechanisms underlying the therapeutic effects of UC-MSCs on renal fibrosis in DN and provide the evidence for DN cell-free therapy based on UC-MSCs in the future.
American Psychological Association (APA)
Li, Hongde& Rong, Pengfei& Ma, Xiaoqian& Nie, Wei& Chen, Yan& Zhang, Juan…[et al.]. 2020. Mouse Umbilical Cord Mesenchymal Stem Cell Paracrine Alleviates Renal Fibrosis in Diabetic Nephropathy by Reducing Myofibroblast Transdifferentiation and Cell Proliferation and Upregulating MMPs in Mesangial Cells. Journal of Diabetes Research،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1183069
Modern Language Association (MLA)
Li, Hongde…[et al.]. Mouse Umbilical Cord Mesenchymal Stem Cell Paracrine Alleviates Renal Fibrosis in Diabetic Nephropathy by Reducing Myofibroblast Transdifferentiation and Cell Proliferation and Upregulating MMPs in Mesangial Cells. Journal of Diabetes Research No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1183069
American Medical Association (AMA)
Li, Hongde& Rong, Pengfei& Ma, Xiaoqian& Nie, Wei& Chen, Yan& Zhang, Juan…[et al.]. Mouse Umbilical Cord Mesenchymal Stem Cell Paracrine Alleviates Renal Fibrosis in Diabetic Nephropathy by Reducing Myofibroblast Transdifferentiation and Cell Proliferation and Upregulating MMPs in Mesangial Cells. Journal of Diabetes Research. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1183069
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1183069