Metformin Improves Epithelial-to-Mesenchymal Transition Induced by TGF-β1 in Renal Tubular Epithelial NRK-52E Cells via Inhibiting Egr-1
Joint Authors
Xu, Lingling
Zheng, Zongji
Guan, Meiping
Xue, Yaoming
Li, Wenqi
Zeng, Yanmei
Wang, Dan
Lyv, Fuping
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The early growth response- (Egr-) 1 has been found to play a key role in organ fibrosis.
Metformin has been shown to be effective in attenuating renal tubular epithelial-to-mesenchymal transition (EMT), which is involved in renal fibrosis.
However, it is unknown whether metformin improves EMT via inhibiting Egr-1.
In this study, rat renal tubular epithelial (NRK-52 E) cells, treated by transforming growth factor- (TGF-) β1 of 10 ng/ml with or without metformin of 1 mmol/l, were transfected by siEgr-1 or M61-Egr-1 plasmids to knock down or overexpress Egr-1, respectively.
The gene and protein expressions of E-cadherin, α-SMA, fibronectin (FN), and Egr-1 were determined by real-time quantitative PCR and Western blotting, respectively.
We observed that TGF-β1 significantly reduced E-cadherin expression and upregulated the expressions of FN, α-SMA, and Egr-1, which can be reversed by metformin.
M61-Egr-1 transfection could exacerbate EMT, which can be reversed by metformin.
Taken together, our data show that Egr-1 plays an important role in TGF-β1-induced EMT of renal tubular epithelial cells and metformin improves EMT while inhibiting Egr-1, which provides a potential novel target to combat renal fibrosis.
American Psychological Association (APA)
Guan, Meiping& Li, Wenqi& Xu, Lingling& Zeng, Yanmei& Wang, Dan& Zheng, Zongji…[et al.]. 2018. Metformin Improves Epithelial-to-Mesenchymal Transition Induced by TGF-β1 in Renal Tubular Epithelial NRK-52E Cells via Inhibiting Egr-1. Journal of Diabetes Research،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1183213
Modern Language Association (MLA)
Guan, Meiping…[et al.]. Metformin Improves Epithelial-to-Mesenchymal Transition Induced by TGF-β1 in Renal Tubular Epithelial NRK-52E Cells via Inhibiting Egr-1. Journal of Diabetes Research No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1183213
American Medical Association (AMA)
Guan, Meiping& Li, Wenqi& Xu, Lingling& Zeng, Yanmei& Wang, Dan& Zheng, Zongji…[et al.]. Metformin Improves Epithelial-to-Mesenchymal Transition Induced by TGF-β1 in Renal Tubular Epithelial NRK-52E Cells via Inhibiting Egr-1. Journal of Diabetes Research. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1183213
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1183213