Uncovering the Mechanism of Astragalus membranaceus in the Treatment of Diabetic Nephropathy Based on Network Pharmacology
Joint Authors
Guo, Ming-Fei
Dai, Ya-Ji
Gao, Jia-Rong
Chen, Pei-Jie
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-06
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Background.
Diabetic nephropathy (DN), characterized by hyperglycemia, hypertension, proteinuria, and edema, is a unique microvascular complication of diabetes.
Traditional Chinese medicine (TCM) Astragalus membranaceus (AM) has been widely used for DN in China while the pharmacological mechanisms are still unclear.
This work is aimed at undertaking a network pharmacology analysis to reveal the mechanism of the effects of AM in DN.
Materials and Methods.
In this study, chemical constituents of AM were obtained via Traditional Chinese Medicine Systems Pharmacology Database (TCMSP), and the potential targets of AM were identified using the Therapeutic Target Database (TTD).
DisGeNET and GeneCards databases were used to collect DN-related target genes.
DN-AM common target protein interaction network was established by using the STRING database.
Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were carried out to further explore the DN mechanism and therapeutic effect of AM.
The network diagrams of the active component-action target and protein-protein interaction (PPI) networks were constructed using Cytoscape software.
Results.
A total of 16 active ingredients contained and 78 putative identified target genes were screened from AM, of which 42 overlapped with the targets of DN and were considered potential therapeutic targets.
The analysis of the network results showed that the AM activity of component quercetin, formononetin, calycosin, 7-O-methylisomucronulatol, and quercetin have a good binding activity with top ten screened targets, such as VEGFA, TNF, IL-6, MAPK, CCL3, NOS3, PTGS2, IL-1β, JUN, and EGFR.
GO and KEGG analyses revealed that these targets were associated with inflammatory response, angiogenesis, oxidative stress reaction, rheumatoid arthritis, and other biological process.
Conclusions.
This study demonstrated the multicomponent, multitarget, and multichannel characteristics of AM, which provided a novel approach for further research of the mechanism of AM in the treatment of DN.
American Psychological Association (APA)
Guo, Ming-Fei& Dai, Ya-Ji& Gao, Jia-Rong& Chen, Pei-Jie. 2020. Uncovering the Mechanism of Astragalus membranaceus in the Treatment of Diabetic Nephropathy Based on Network Pharmacology. Journal of Diabetes Research،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1183234
Modern Language Association (MLA)
Guo, Ming-Fei…[et al.]. Uncovering the Mechanism of Astragalus membranaceus in the Treatment of Diabetic Nephropathy Based on Network Pharmacology. Journal of Diabetes Research No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1183234
American Medical Association (AMA)
Guo, Ming-Fei& Dai, Ya-Ji& Gao, Jia-Rong& Chen, Pei-Jie. Uncovering the Mechanism of Astragalus membranaceus in the Treatment of Diabetic Nephropathy Based on Network Pharmacology. Journal of Diabetes Research. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1183234
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1183234