Exploring the Mechanism of Skeletal Muscle in a Tacrolimus-Induced Posttransplantation Diabetes Mellitus Model on Gene Expression Profiles

Joint Authors

Chen, Bi-Cheng
Li, Ding
Sun, Linxiao
Zheng, Chenlei
Wang, Cheng
Zhang, Tan
Ni, Xiao-feng
Lin, Jian-Hu

Source

Journal of Diabetes Research

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-01-10

Country of Publication

Egypt

No. of Pages

11

Main Subjects

Diseases
Medicine

Abstract EN

Objective.

Posttransplantation diabetes mellitus (PTDM) is a known complication of transplantation that affects the prognosis.

Tacrolimus (Tac or FK506) is a widely used immunosuppressant that has been reported to be a risk factor for PTDM and to further induce complications in heart and skeletal muscles, but the mechanism is still largely unknown.

In our preliminary experiments, we found that after Tac treatment, blood glucose increased, and the weight of skeletal muscle declined.

Here, we hypothesize that tacrolimus can induce PTDM and influence the atrophy of skeletal muscle.

Methods.

We designed preliminary experiments to establish a tacrolimus-induced PTDM model.

Gene expression profiles in quadriceps muscle from this rat model were characterized by oligonucleotide microarrays.

Then, differences in gene expression profiles in muscle from PTDM rats that received tacrolimus and control subjects were analyzed by using GeneSpring GX 11.0 software (Agilent).

Functional annotation and enrichment analysis of differentially expressed genes (DEGs) helped us identify clues for the side effects of tacrolimus.

Results.

Our experiments found that the quadriceps in tacrolimus-induced PTDM group were smaller than those in the control group.

The study identified 275 DEGs that may be responsible for insulin resistance and the progression of PTDM, including 86 upregulated genes and 199 downregulated genes.

GO and KEGG functional analysis of the DEGs showed a significant correlation between PTDM and muscle development.

PPI network analysis screened eight hub genes and found that they were related to troponin and tropomyosin.

Conclusions.

This study explored the molecular mechanism of muscle atrophy in a tacrolimus-induced PTDM model by bioinformatics analyses.

We identified 275 DEGs and identified significant biomarkers for predicting the development and progression of tacrolimus-induced PTDM.

American Psychological Association (APA)

Zheng, Chenlei& Wang, Cheng& Zhang, Tan& Li, Ding& Ni, Xiao-feng& Lin, Jian-Hu…[et al.]. 2020. Exploring the Mechanism of Skeletal Muscle in a Tacrolimus-Induced Posttransplantation Diabetes Mellitus Model on Gene Expression Profiles. Journal of Diabetes Research،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1183292

Modern Language Association (MLA)

Zheng, Chenlei…[et al.]. Exploring the Mechanism of Skeletal Muscle in a Tacrolimus-Induced Posttransplantation Diabetes Mellitus Model on Gene Expression Profiles. Journal of Diabetes Research No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1183292

American Medical Association (AMA)

Zheng, Chenlei& Wang, Cheng& Zhang, Tan& Li, Ding& Ni, Xiao-feng& Lin, Jian-Hu…[et al.]. Exploring the Mechanism of Skeletal Muscle in a Tacrolimus-Induced Posttransplantation Diabetes Mellitus Model on Gene Expression Profiles. Journal of Diabetes Research. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1183292

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1183292