L-Carnitine: An Antioxidant Remedy for the Survival of Cardiomyocytes under Hyperglycemic Condition
Joint Authors
Luzi, Livio
Montesano, Anna
Terruzzi, Ileana M.
Senesi, Pamela
Vacante, Fernanda
Frigerio, Alice
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-09
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background.
Metabolic alterations as hyperglycemia and inflammation induce myocardial molecular events enhancing oxidative stress and mitochondrial dysfunction.
Those alterations are responsible for a progressive loss of cardiomyocytes, cardiac stem cells, and consequent cardiovascular complications.
Currently, there are no effective pharmacological measures to protect the heart from these metabolic modifications, and the development of new therapeutic approaches, focused on improvement of the oxidative stress condition, is pivotal.
The protective effects of levocarnitine (LC) in patients with ischemic heart disease are related to the attenuation of oxidative stress, but LC mechanisms have yet to be fully understood.
Objective.
The aim of this work was to investigate LC’s role in oxidative stress condition, on ROS production and mitochondrial detoxifying function in H9c2 rat cardiomyocytes during hyperglycemia.
Methods.
H9c2 cells in the hyperglycemic state (25 mmol/L glucose) were exposed to 0.5 or 5 mM LC for 48 and 72 h: LC effects on signaling pathways involved in oxidative stress condition were studied by Western blot and immunofluorescence analysis.
To evaluate ROS production, H9c2 cells were exposed to H2O2 after LC pretreatment.
Results.
Our in vitro study indicates how LC supplementation might protect cardiomyocytes from oxidative stress-related damage, preventing ROS formation and activating antioxidant signaling pathways in hyperglycemic conditions.
In particular, LC promotes STAT3 activation and significantly increases the expression of antioxidant protein SOD2.
Hyperglycemic cardiac cells are characterized by impairment in mitochondrial dysfunction and the CaMKII signal: LC promotes CaMKII expression and activation and enhancement of AMPK protein synthesis.
Our results suggest that LC might ameliorate metabolic aspects of hyperglycemic cardiac cells.
Finally, LC doses herein used did not modify H9c2 growth rate and viability.
Conclusions.
Our novel study demonstrates that LC improves the microenvironment damaged by oxidative stress (induced by hyperglycemia), thus proposing this nutraceutical compound as an adjuvant in diabetic cardiac regenerative medicine.
American Psychological Association (APA)
Vacante, Fernanda& Senesi, Pamela& Montesano, Anna& Frigerio, Alice& Luzi, Livio& Terruzzi, Ileana M.. 2018. L-Carnitine: An Antioxidant Remedy for the Survival of Cardiomyocytes under Hyperglycemic Condition. Journal of Diabetes Research،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1183504
Modern Language Association (MLA)
Vacante, Fernanda…[et al.]. L-Carnitine: An Antioxidant Remedy for the Survival of Cardiomyocytes under Hyperglycemic Condition. Journal of Diabetes Research No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1183504
American Medical Association (AMA)
Vacante, Fernanda& Senesi, Pamela& Montesano, Anna& Frigerio, Alice& Luzi, Livio& Terruzzi, Ileana M.. L-Carnitine: An Antioxidant Remedy for the Survival of Cardiomyocytes under Hyperglycemic Condition. Journal of Diabetes Research. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1183504
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1183504