Probing the Effect of Ag2S Quantum Dots on Human Serum Albumin Using Spectral Techniques
Joint Authors
Chen, Lu
Fu, Yiying
Guan, Enli
Ren, Guolan
Liang, Jiangong
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-06
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The understanding of the interaction between protein and quantum dots (QDs) has significant implications for biological applications of QDs.
Herein, we studied the effect of Ag2S QDs on human serum albumin (HSA) using UV-Vis absorption spectra and fluorescence spectroscopy and found that the fluorescence intensity of HSA was gradually decreased with increasing Ag2S QDs concentrations.
By using the Stern-Volmer equation for the fluorescence quenching constant (KSV) of the response of Ag2S QDs to HSA as well as thermodynamic equations, the values of thermodynamic enthalpy change (ΔHθ), entropy change (ΔSθ), and free energy change (ΔGθ) were calculated to be −10.79 KJ·mol−1, 37.80 J·mol−1·K−1, and −22.27 KJ·mol−1, respectively.
The results indicate that Ag2S QDs exert an obvious static fluorescence quenching effect on HSA and electrostatic interaction plays a key role in the binding process.
Furthermore, Raman spectral analysis reveals that Ag2S QDs alter the external environment of tyrosine and tryptophan or the C-H bending of HSA but not the α-helical content.
American Psychological Association (APA)
Fu, Yiying& Guan, Enli& Liang, Jiangong& Ren, Guolan& Chen, Lu. 2017. Probing the Effect of Ag2S Quantum Dots on Human Serum Albumin Using Spectral Techniques. Journal of Nanomaterials،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1183513
Modern Language Association (MLA)
Fu, Yiying…[et al.]. Probing the Effect of Ag2S Quantum Dots on Human Serum Albumin Using Spectral Techniques. Journal of Nanomaterials No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1183513
American Medical Association (AMA)
Fu, Yiying& Guan, Enli& Liang, Jiangong& Ren, Guolan& Chen, Lu. Probing the Effect of Ag2S Quantum Dots on Human Serum Albumin Using Spectral Techniques. Journal of Nanomaterials. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1183513
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1183513