Xuesaitong Protects Podocytes from Apoptosis in Diabetic Rats through Modulating PTEN-PDK1-Akt-mTOR Pathway
Joint Authors
Yang, Xifei
Chen, Teng
Xu, Youhua
Zhai, Ruonan
Jian, Guihua
Xie, Ling
Xue, Rui
Wang, Niansong
Gui, Dingkun
Zheng, Zening
Su, Jun
Gao, Chongting
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-21
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Diabetic kidney disease (DKD) is a major cause of end-stage renal disease (ESRD), and therapeutic strategies for delaying its progression are limited.
Loss of podocytes by apoptosis characterizes the early stages of DKD.
To identify novel therapeutic options, we investigated the effects of Xuesaitong (XST), consisting of total saponins from Panax notoginseng, on podocyte apoptosis in streptozotocin- (STZ-) induced diabetic rats.
XST (5 mg/kg·d) or Losartan (10 mg/kg·d) was given to diabetic rats for 12 weeks.
Albuminuria, renal function markers, and renal histopathology morphological changes were examined.
Podocyte apoptosis was determined by triple immunofluorescence labelling including a TUNEL assay, WT1, and DAPI.
Renal expression of Nox4, miRNA-214, PTEN, PDK1, phosphorylated Akt, mTOR, and mTORC1 was detected.
In diabetic rats, severe hyperglycaemia and albuminuria developed, and apoptotic podocytes were markedly increased in diabetic kidneys.
However, XST attenuated albuminuria, mesangial expansion, podocyte apoptosis, and morphological changes of podocytes in diabetic rats.
Decreased expression of PTEN, as well as increased expression of Nox4, miRNA-214, PDK1, phosphorylated Akt, mTOR, and mTORC1, was detected.
These abnormalities were partially restored by XST treatment.
Thus, XST ameliorated podocyte apoptosis partly through modulating the PTEN-PDK1-Akt-mTOR pathway.
These novel findings might point the way to a natural therapeutic strategy for treating DKD.
American Psychological Association (APA)
Xue, Rui& Zhai, Ruonan& Xie, Ling& Zheng, Zening& Jian, Guihua& Chen, Teng…[et al.]. 2020. Xuesaitong Protects Podocytes from Apoptosis in Diabetic Rats through Modulating PTEN-PDK1-Akt-mTOR Pathway. Journal of Diabetes Research،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1183543
Modern Language Association (MLA)
Xue, Rui…[et al.]. Xuesaitong Protects Podocytes from Apoptosis in Diabetic Rats through Modulating PTEN-PDK1-Akt-mTOR Pathway. Journal of Diabetes Research No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1183543
American Medical Association (AMA)
Xue, Rui& Zhai, Ruonan& Xie, Ling& Zheng, Zening& Jian, Guihua& Chen, Teng…[et al.]. Xuesaitong Protects Podocytes from Apoptosis in Diabetic Rats through Modulating PTEN-PDK1-Akt-mTOR Pathway. Journal of Diabetes Research. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1183543
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1183543